A biosensor for detecting Salmonella typhimurium and its preparation method

A biosensor, Salmonella technology, applied in the field of biosensors, can solve the problems of high cost, low specificity and sensitivity, achieve low detection limit, simple preparation method, and improve the effect of reaction speed

Inactive Publication Date: 2017-03-29
UNIV OF JINAN
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of relatively low specificity and sensitivity and high cost in the method for detecting Salmonella typhimurium, the present invention provides a method for detecting Salmonella typhimurium based on nucleic acid aptamers with high specificity and sensitivity, low cost and fast detection speed biosensor

Method used

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  • A biosensor for detecting Salmonella typhimurium and its preparation method

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Embodiment 1

[0051] as attached figure 1 As shown, a biosensor for detecting Salmonella typhimurium includes a gold electrode 1, and a capture probe layer 2 and an RCA product layer 3 are sequentially modified on the gold electrode 1 from the inside to the outside.

[0052] The preparation method of the biosensor provided by the present invention is as follows:

[0053] a. The gold electrode 1 is first polished in 0.3 and 0.05 μm alumina slurry until it becomes a mirror surface, and rinsed repeatedly with PBS and secondary water;

[0054] b. Add 10 μL of capture probe (10 μM) dropwise to the electrode surface, incubate for 2 h at room temperature, and fix the thiol chain to the electrode surface through Au-S bond; the modification process of the capture probe layer 2 of the electrode has come to an end. , and then modify the RCA product layer 3 in the homogeneous solution. The modification steps in the homogeneous reaction are as follows:

[0055] a. Add sterilized water, an appropriate...

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Abstract

The invention provides a biosensor for detecting salmonella typhimurium, which comprises a gold electrode, and a capture probe layer and a RCA product layer, which successively modify the gold electrode externally. The preparation method is carried out through specificity recognition of an aptamer. The produced electrode is high in sensitivity and specificity and can be used for quickly detecting the salmonella typhimurium.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting Salmonella typhimurium based on nucleic acid aptamers, and a preparation method thereof. Background technique [0002] Salmonella is one of the important food-borne pathogens of Enterobacteriaceae, and it occupies a high proportion of food poisoning cases in my country. Salmonella typhimurium has attracted widespread attention due to its strong drug resistance, wide transmission routes, and many infecting hosts. Studies have shown that Salmonella typhimurium has strong multi-drug resistance, showing resistance to most antibiotics. In the survey, the number of Salmonella typhimurium infections accounted for 17% of the total number of Salmonella infections that year. At the same time, among more than 290 Salmonella serotypes found in my country, Salmonella typhimurium is also one of the most common serotypes. [0003] At present, the main methods use...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12Q1/68
CPCG01N27/3275G01N33/56916G01N2333/255Y02A50/30
Inventor王玉邱婷婷黄加栋刘素徐伟王虹智郭玉娜许颖崔洁
OwnerUNIV OF JINAN