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Biosensor for detecting salmonella typhimurium and preparation method of same

A biosensor, Salmonella technology, applied in the field of biosensors, can solve the problems of high cost, low specificity and sensitivity, and achieve the effects of low detection limit, simple preparation method and reduced complexity

Inactive Publication Date: 2016-01-13
UNIV OF JINAN
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  • Claims
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Problems solved by technology

[0004] In order to solve the problems of relatively low specificity and sensitivity and high cost in the method for detecting Salmonella typhimurium, the present invention provides a method for detecting Salmonella typhimurium based on nucleic acid aptamers with high specificity and sensitivity, low cost and fast detection speed biosensor

Method used

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  • Biosensor for detecting salmonella typhimurium and preparation method of same

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Embodiment 1

[0051] as attached figure 1 As shown, a biosensor for detecting Salmonella typhimurium includes a gold electrode 1, on which a captureprobe layer 2 and an RCA product layer 3 are sequentially decorated from inside to outside.

[0052] The preparation method of the biosensor provided by the invention is as follows:

[0053] a. Gold electrode 1 is firstly polished in 0.3 and 0.05 μm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;

[0054] b. Drop 10 μL of captureprobe (10 μM) onto the surface of the electrode, incubate at room temperature for 2 hours, and fix the sulfhydryl chains on the surface of the electrode through the Au-S bond; RCA product layer 3 is modified in the phase solution, and the modification steps in the homogeneous reaction are as follows:

[0055] a. Add sterilized water, 10× appropriate amount of buffer (buffer), 1 μL HAP DNA chain (10 μM) and 1 μL different concentrations of target (100pM, 500pM,...

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Abstract

The invention provides a biosensor for detecting salmonella typhimurium, which comprises a gold electrode, and a capture probe layer and a RCA product layer, which successively modify the gold electrode externally. The preparation method is carried out through specificity recognition of an aptamer. The produced electrode is high in sensitivity and specificity and can be used for quickly detecting the salmonella typhimurium.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting Salmonella typhimurium based on nucleic acid aptamers, and also relates to a preparation method thereof. Background technique [0002] Salmonella is one of the important food-borne pathogens of Enterobacteriaceae, which accounts for a relatively high proportion of food poisoning cases in my country. Salmonella typhimurium has been widely concerned because of its strong drug resistance, wide transmission route, and multiple host infections. Studies have shown that Salmonella typhimurium has extremely strong multi-drug resistance, showing resistance to most antibiotics. In the survey, the number of Salmonella typhimurium infections accounted for 17% of the total Salmonella infections that year. At the same time, Salmonella typhimurium is also one of the most common serotypes among more than 290 serotypes of Salmonella found in my country. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N27/327
CPCG01N27/3275G01N33/56916G01N2333/255Y02A50/30
Inventor 王玉邱婷婷黄加栋刘素徐伟王虹智郭玉娜许颖崔洁
Owner UNIV OF JINAN
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