Application of TMF1 as Parkinsonism diagnosis marker

A Parkinson's disease and product technology, applied in the field of molecular diagnosis, can solve problems such as unstable posture, muscle stiffness, gait disorder, etc., and achieve more accurate genetic diagnosis, reduce mortality, and timely genetic diagnosis

Inactive Publication Date: 2016-03-09
周丽丽 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. Muscle stiffness
[0006] 3. Slow movement
[0007] 4. Unstable posture
[0008] 5. Dysgraphia and gait disturbance
[0009] The methods for diag...

Method used

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  • Application of TMF1 as Parkinsonism diagnosis marker
  • Application of TMF1 as Parkinsonism diagnosis marker
  • Application of TMF1 as Parkinsonism diagnosis marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Screening for genes differentially expressed in Parkinson's disease patients and normal individuals

[0051] 1. Research object:

[0052] Collect 10 cases of primary PD patients, 5 males and 5 females, aged 30-85 years, with a course of disease ranging from 3 months to 22 years. PD inclusion criteria: all diagnostic criteria meet the clinical diagnostic criteria for PD (refer to "Jiang Yuping, Wang Jian, Ding Zhengtong, et al., Diagnostic criteria for primary Parkinson's disease, 2005, Chinese Clinical Neuroscience, 2006, 14:40" ). Exclusion criteria: (1) essential tremor; (2) secondary parkinsonism; (3) severe dementia, dysarthria; (4) patients with other mental diseases. This study was approved by the Hospital Ethics Committee and all patients signed informed consent.

[0053] Normal group: select 10 healthy volunteers aged 30-85, 5 males and 5 males.

[0054] There was no significant difference in age and gender between the two groups (P>0.10), which we...

Embodiment 2

[0080] Embodiment 2QPCR experiment verifies the genes differentially expressed in patients with Parkinson's disease and normal people

[0081] 1. Research object:

[0082] The screening criteria were the same as in Example 1, 50 cases of Parkinson's disease patients and 50 normal persons.

[0083] 2. Extraction of total RNA in blood

[0084] Step is with embodiment 1.

[0085] 3. Reverse transcription

[0086]1 μg of total RNA was reverse-transcribed to synthesize cDNA using reverse transcription buffer. Use 25μl reaction system, take 1μg total RNA for each sample as template RNA, and add the following components to the PCR tube: DEPC water, 5× reverse transcription buffer, 10mmol / LdNTP, 0.1mmol / lDTT, 30μmmol / lOligodT, 200U / μl M-MLV, template RNA. Incubate at 42°C for 1 hour, then centrifuge briefly at 72°C for 10 minutes.

[0087] 4. QPCR

[0088] (1) Primer design

[0089] QPCR amplification primers were designed according to the coding sequences of TMF1 gene and GA...

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Abstract

The invention discloses TMF1 genes which can be used as molecular markers for early diagnosis of Parkinsonism. A gene chip and a QPCR method are adopted to find that the TMF1 genes are remarkably different in expression in blood of a normal person and in blood of a patient with the Parkinsonism, that is, whether an object suffers from the Parkinsonism or not can be judged by detecting expression of the TMF1 genes in blood. According to relativity between expression of the TMF1 genes and the Parkinsonism, the invention discloses a kit for Parkinsonism diagnosis. The kit is used for diagnosing the Parkinsonism by detecting expression of the TMF1 genes. The diagnosis kit can be used for early diagnosis of the disease and has a wide application prospect clinically.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to a molecular marker for the diagnosis of Parkinson's disease, in particular to the application of the molecular marker-TMF1 gene in blood in the preparation of products for diagnosing Parkinson's disease. Background technique [0002] Parkinson's disease (Parkinson's disease, PD) is a common neurodegenerative disease, more common in the elderly, the average age of onset is about 60 years old, young Parkinson's disease onset under the age of 40 is rare. The prevalence of PD in people over 65 years old in my country is about 1.7%. Most Parkinson's disease patients are sporadic cases, and less than 10% of patients have a family history. The main pathological change of Parkinson's disease is the degeneration and death of dopamine (DA) neurons in the substantia nigra of the midbrain, which leads to a significant decrease in the content of DA in the striatum and causes the disease. Th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/2835
Inventor 周丽丽孙梅芬
Owner 周丽丽
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