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A kind of wettable powder of Streptomyces aureus verticalus hn6

A technology of wettable powder and streptomyces, applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of spore suspension and liquid fermented products that are not resistant to storage and unsuitable for transportation, so as to achieve good control effect and ensure quality The effect with safety and remarkable effect

Active Publication Date: 2018-03-23
HAINAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the research and application of this strain mainly used spore suspension or liquid fermented product, but both spore suspension and liquid fermented product are not suitable for storage and transportation, which will inevitably limit the large-scale application of this strain

Method used

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  • A kind of wettable powder of Streptomyces aureus verticalus hn6
  • A kind of wettable powder of Streptomyces aureus verticalus hn6
  • A kind of wettable powder of Streptomyces aureus verticalus hn6

Examples

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Embodiment 1

[0017] The preparation method of embodiment 1 Streptomyces aureus verticalus HN6 wettable powder

[0018] (1) Strain activation:

[0019] The strain used in the present invention is Streptomyces aureoverticillatus HN6 (Streptomyces aureoverticillatus HN6). The bacteria were inoculated in the expansion medium (soluble starch 2%, KNO 3 0.1%, K 2 HPO 4 0.05%, MgSO 4 0.05%, NaCl 0.05%, FeSO 4 0.001%, 2% agar, replenish water to 100%, after the drug is completely dissolved, adjust the pH to 7.0-7.2, sterilize at 121° C. for 20 minutes by high temperature and high pressure) on a solid plate, and culture at a constant temperature of 28° C. for 5 days.

[0020] (2) Primary seed preparation:

[0021] The concentration of activated Streptomyces aureus verticalus HN6 was 10 9 cfu g -1 The spore suspension was then inoculated in a 5% inoculum with a primary seed culture solution (soluble starch 2%, KNO 3 0.1%, K 2 HPO 4 0.05%, MgSO 4 0.05%, NaCl 0.05%, FeSO 4 0.001%, a...

example 10

[0026] Example 1 The preparation method of 0.1 billion / gram Streptomyces aureus verticalus HN6 wettable powder

[0027] It is made of 5% Streptomyces aureus verticalus liquid fermentation product, 1% sodium lauryl sulfate, 5% NNO, 2% potassium dihydrogen phosphate and 87% diatomaceous earth. According to the above weight, put it into a jet mill for pulverization, pass through a 325 mesh sieve, the moisture content is less than or equal to 5%, and the active spores are detected to be 0.1 billion / g. After passing the inspection, it is packaged and stored.

example 23

[0028] The preparation method of example 2 300,000,000 / gram Streptomyces aureus verticalus HN6 wettable powder

[0029]It is made of 10% of Streptomyces aureus verticalus liquid fermentation product, 5% of sodium methylene bis-naphthalene sulfonate, 1% of NNO, 1% of polyaspartic acid, 0.5% of vitamin E and 82.5% of bentonite. According to the above weight, put it into a jet mill for pulverization, pass through a 325-mesh sieve, the moisture content is ≤5%, and the active spores are detected to be 300 million / g. After passing the inspection, it is packaged and stored.

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Abstract

The invention discloses a wettable powder of Streptomyces aureus verticalus HN6, which is prepared from the following components in mass percentage: 5%-20% of liquid fermentation product of Streptomyces aureus verticalis HN6, 1%-5% of wetting agent, dispersing 1% to 5% of the agent, 0.5% to 2% of the stabilizer, and 100% of the filler. The preparation method includes: putting wetting agent, dispersant, stabilizer and filler into the airflow mill according to the above weight to pulverize, passing through a 325-mesh sieve, and after cooling down, adding the liquid fermentation product of Streptomyces aureus verticalus HN6, and continuing pulverization, the moisture content is ≤ 5%, the number of active spores is 0.1 to 1 billion / g, which is qualified after inspection and packed for storage. Beneficial effects of the present invention: the medicament belongs to living microorganism preparation, has remarkable effect on banana wilt, has good control effect on other diseases, has no toxic and side effects on ecological environment, and can guarantee the quality and safety of agricultural products.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a wettable powder of Streptomyces aureus verticalus HN6. Background technique [0002] Banana Fusarium wilt, also known as Panama disease, is caused by the infection of Fusarium oxysporumf.sp.cubeme FOC. It is an international quarantine object. When the disease is severe, it can lead to the loss of banana orchards and seriously affect banana planting. industry development. In recent years, the negative impacts caused by chemical pesticides, such as pesticide residues and damage to the ecological environment, have become increasingly serious, and the use of biocontrol microorganisms to control crop diseases has gradually attracted attention. Actinomycetes are an extremely important group of bacteria in biocontrol microorganisms, and have been widely used in the field of agricultural biological control. The application of bio-control actinomycetes mainly has two as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N25/14A01P3/00
Inventor 骆焱平王兰英
Owner HAINAN UNIV
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