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High-concentration NAA and glycerol gel root soaking method

A glycerin gel, high-concentration technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, unsatisfactory production effect, etc., and achieve the effect of reducing water evaporation and easy operation

Inactive Publication Date: 2016-03-23
何晴雪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] ① Hormone mother solution preparation: Accurately weigh 0.1g of NAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL NAA mother solution;

[0014] ②Preparation of working solution: Dilute the above mother solution into a high-concentration hormone working solution with a concentration of 500mg / L according to the volume ratio of 1:2, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0015] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone and glycerin working solution with a concentration of 500mg / L in a water bath at 50°C for later use;

[0016] Root dipping: Dip the lower end of the 8-10cm long, healthy and strong boxwood branch in the above gel sealing solution for 3-5 seconds and take it out;

[0017] Cuttings: Insert the above-mentioned branches into the cultivation medium to take root.

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Abstract

The invention discloses a high-concentration NAA and glycerol gel root soaking method and belongs to the technical field of plant biotechnology. Firstly, mother liquor with the concentration being 1 mg / mL is prepared; the mother liquor is diluted into high-concentration NAA work liquid with the concentration being 500 mg / L; the work liquid is used for preparing high-concentration NAA and glycerol gel closing liquid with the mass concentration of sodium carboxymethylcellulose being 1% and the mass concentration of sodium alginate being 8%; the lower ends of healthy, sturdy branches are soaked in the gel confining liquid for 3-5 seconds and taken out; the branches are inserted into culture media to root.

Description

technical field [0001] The invention discloses a root dipping method of high-concentration NAA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N37/10A01P21/00A01G9/10A01G7/06
CPCA01N25/04A01G7/06A01G9/0299A01N37/10
Inventor 何晴雪
Owner 何晴雪