Culture method of brachytheciaceae

A cultivation method and technology of moss, applied in the fields of reproduction and cultivation of bryophytes, can solve the problems of time-consuming and labor-consuming, and achieve the effects of low cost, simple operation and fast growth

Inactive Publication Date: 2016-04-13
CHONGQING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing technology mainly uses tissue culture for propagation, which has high requirements for experimental equipment, experimental environment and operato

Method used

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  • Culture method of brachytheciaceae
  • Culture method of brachytheciaceae
  • Culture method of brachytheciaceae

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with embodiment.

[0013] The instruments, reagents, materials, etc. involved in the following examples, unless otherwise specified, are conventional instruments, reagents, materials, etc. in the prior art, and can be obtained through formal commercial channels. The experimental methods, detection methods, etc. involved in the following examples, unless otherwise specified, are conventional experimental methods, detection methods, etc. in the prior art.

[0014] Screening of the Best Culture Method of Experimental Moss

[0015] 1. Method

[0016] 1.1 Preparation of culture medium

[0017] According to the formulations of Beneck, MS and Knop nutrient solutions (both are well-known media in the prior art), 3 L of nutrient solutions with a concentration of 1X were first prepared. Then take 500mL of 1X concentration of Knop culture solution, dilute it with distilled water, and prepare it to 1L. At this ...

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Abstract

The invention discloses a culture method of brachytheciaceae. The culture method comprises the following steps: picking up brachytheciaceae growing in the field; spreading the brachytheciaceae on a culture substrate, wherein the culture substrate is obtained by mixing vermiculite and pearlite according to the volume ratio of 1:1; putting the spread brachytheciaceae at a place where the sun cannot shine on the spread brachytheciaceae directly; carrying out culture at the room temperature while keeping the humidity of 70-80%; pouring a nutrient solution every other day, wherein the pouring quantity is 1 to 2 ml/cm<2>, the nutrient solution is a Knop nutrient solution of which the concentration is 1/8, and the pH value of the nutrient solution is 6.0 to 6.5; pouring water every third day, wherein the pouring quantity is 2 to 4 ml/cm<2>. According to the culture method, the brachytheciaceae growing in the field is transplanted to the most proper substrate, the most proper nutrient solution is sprayed on the transplanted brachytheciaceae periodically, and the transplanted brachytheciaceae propagates indoor, so that the transplanted brachytheciaceae grows fast; no special requirements on experiment equipment are required; the operation is simple; the cost is low; popularization and application of brachytheciaceae are facilitated.

Description

technical field [0001] The invention relates to a method for cultivating mosses, belonging to the technical field of cultivation and propagation of bryophytes. Background technique [0002] At present, bryophytes are widely used in landscaping, especially moss micro-landscapes are becoming more and more popular, and there is a great demand for bryophytes. Moss (scientific name: Brachytheciumalbicans) belongs to the lateral capsular moss, which grows in flakes, like a green lawn, which can beautify the environment and contain water. The existing technology mainly uses tissue culture for propagation, which has high requirements for experimental equipment, experimental environment, and operator's experimental technology. Often, bacteria are infected because the experimenter does not operate properly, and all previous efforts are wasted, time-consuming and labor-intensive. Contents of the invention [0003] Aiming at the above-mentioned prior art, the present invention provid...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G31/00A01G24/00A01G24/35
Inventor 刘艳
Owner CHONGQING NORMAL UNIVERSITY
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