Fiber strength related molecular mark from gossypium barbadense and application thereof

A molecular marker, fiber strength technology, applied in application, microbial determination/inspection, DNA/RNA fragment, etc., can solve the problems of slow progress, poor accuracy and low efficiency of fiber quality breeding, saving costs and solving slow progress , the effect of speeding up the cultivation process

Active Publication Date: 2016-04-13
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome many shortcomings such as poor accuracy of phenotypic selection, long cycle, and low efficiency in traditional breeding, and solve the problem of slow progress in fiber quality breeding, the present invention provides a cotton fiber strength QTL / principal Molecular markers linked to effective genes, that is, by screening molecular markers linked to fiber strength QTL / main effect genes from Sea Island Cotton Sea 1, a high-quality fiber material, to perform early marker-assisted selection at the DNA level and improve breeding efficiency

Method used

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  • Fiber strength related molecular mark from gossypium barbadense and application thereof
  • Fiber strength related molecular mark from gossypium barbadense and application thereof
  • Fiber strength related molecular mark from gossypium barbadense and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening Molecular Markers

[0048] (1) Acquisition of introgression lines and their phenotype data

[0049] Using the early-maturing upland cotton Zhongmian 36 as the recipient parent and Haidao Mianhai 1 as the donor parent, a land-sea hybrid combination was assembled, and Zhongmian 36 was used as the recurrent parent for multi-generation backcrossing, self-crossing, and SSR molecular markers Identification and construction of a set of introgression materials for land and sea chromosome fragments. The inventor planted 2660 individual plants in the experimental farm of China Cotton Research Institute in Anyang, Henan in 2009, and selected 658 BC from them in 2010 5 f 3 A single plant with high fiber quality was planted in the experimental farm of China Cotton Research Institute in Anyang, Henan, and obtained 658 BC 5 f 3:4 Plant line. From the 658 plant rows, randomly screened the stable high-quality fiber plant rows, and planted them in 2011 (BC 5 f ...

Embodiment 2

[0080] Example 2 Molecular marker selection method for improving fiber strength traits of upland cotton

[0081] Using the molecular marker HAU0883 obtained in Example 1 125 and TMB1125 220 Molecular marker selection is carried out in the breeding population related to Sea Island Cotton Sea 1, etc., including the following steps:

[0082] (1) DNA extraction: take Sea Island Cotton Sea 1 as the donor parent, and upland cotton varieties or strains (such as Lumianyan 28 and Zhongmian Institute 60) as the recipient parent, perform hybridization and backcrossing to obtain segregated populations, or use Haidao Mianhai 1 is the donor parent, and the upland cotton varieties (such as Lumianyan 28 and Zhongmiansuo 60) are the introgression lines and their derivatives obtained by crossing the high-generation backcross of the recipient parents, or the introgression lines and the land cotton varieties. The offspring populations of hybridization and backcrossing of cotton varieties, etc.,...

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Abstract

The invention relates to the field of molecular breeding, and particularly relates to a fiber strength related molecular mark from gossypium barbadense and application thereof. The molecular mark is HAU0883125 and TMB1125220, wherein the HAU0883125 is closely linked with the site qFS-C14-1 of cotton fiber strength QTL, and the TMB1125220 is closely linked with the site qFS-C20-1 of cotton fiber strength QTL; and the qFS-C14-1 is located on chromosome C14, and the qFS-C20-1 is located on chromosome C20. The molecular mark can be used for improving the selection efficiency of fiber strength, overcoming the defect in existing breeding technology for fiber quality identification and accelerating the culturing progress of high-quality new varieties.

Description

technical field [0001] The invention relates to the technical field of cotton molecular breeding, in particular to a molecular marker related to fiber strength from Sea Island Cotton Sea 1 and its application. Background technique [0002] Cotton is an important economic crop in the world, and cotton fiber is an important raw material for textile industry. Cotton occupies an important position in my country's national economy. With the rapid development of the textile industry and the continuous improvement of people's living standards, the requirements for the quality of cotton fibers are also getting higher and higher. At present, the main problems of cotton varieties in my country are poor fiber quality and narrow genetic basis. Simultaneous improvement of fiber quality and yield is the main goal of current cotton breeding. Fiber strength is an important indicator of fiber quality traits. Cultivating new cotton varieties with high fiber strength is an important require...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 石玉真袁友禄黎波涛刘爱英龚举武李俊文宋威武巩万奎商海红陈婷婷葛群
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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