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Extracting method for stevioside in stevia rebaudiana

A technology of steviol glycosides and extraction methods, which is applied in the field of natural product extraction, and can solve problems such as cumbersome overall processes

Active Publication Date: 2016-05-04
SEPAX TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a method for extracting steviol glycosides in stevia rebaudiana with a simple process for the problem of cumbersome overall process in the traditional extraction method of steviol glycosides in stevia rebaudiana

Method used

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  • Extracting method for stevioside in stevia rebaudiana

Examples

Experimental program
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Effect test

Embodiment 1

[0041]Mix 25g of stevia dry leaves with 200g of pure water for leaching, maintain the leaching temperature at 90°C, and leaching time for 40min, and then perform solid-liquid separation to obtain solid and liquid. Continue to mix the above solid with 200g of pure water, repeat the above leaching operation three times, keep the liquid after each leaching to obtain an extract, and finally add ethanol to make the ethanol ratio in the extract reach 10%. Perform HPLC (high performance liquid chromatography) test on the extract, such as figure 2 As shown, the results show that except for steviol glycosides St, RA and Rc in the extract, the peaks of other impurities are very high, indicating that the content of impurities in the extract is very high.

[0042] The above leaching solution was loaded onto a solid-phase extraction column packed with 30g of Generik RP filler equilibrated with 10% methanol aqueous solution in advance, and then the pretreatment eluent, the first target elu...

Embodiment 2

[0045] Mix 25g of stevia dry leaves with 200g of pure water for leaching, maintain the leaching temperature at 90°C, and leaching time for 40min, and then perform solid-liquid separation to obtain solid and liquid. Continue to mix the above solid with 200g of pure water, repeat the above leaching operation three times, keep the liquid after each leaching to obtain an extract, and finally add ethanol to make the ethanol ratio in the extract reach 10%. Perform HPLC (high performance liquid chromatography) test on the extract, such as figure 2 As shown, the results show that except for steviol glycosides St, RA and Rc in the extract, the peaks of other impurities are very high, indicating that the content of impurities in the extract is very high.

[0046] The above extracts were loaded onto the FLASH column packed with 30g of GenerikRP filler equilibrated with 10% methanol aqueous solution in advance, and then the pretreatment eluent, the first target eluate and the second targ...

Embodiment 3

[0049] Mix 25g of stevia dry leaves with 200g of pure water for leaching, maintain the leaching temperature at 90°C, and leaching time for 40min, and then perform solid-liquid separation to obtain solid and liquid. Continue to mix the above solid with 200g of pure water, repeat the above leaching operation three times, keep the liquid after each leaching to obtain an extract, and finally add ethanol to make the ethanol ratio in the extract reach 10%. Perform HPLC (high performance liquid chromatography) test on the extract, such as figure 2 As shown, the results show that except for steviol glycosides St, RA and Rc in the extract, the peaks of other impurities are very high, indicating that the content of impurities in the extract is very high.

[0050] The above leaching solution was loaded onto a liquid chromatography column packed with 30g of GenerikRP filler equilibrated with 10% aqueous methanol solution in advance, and then the pretreatment eluent, the first target elue...

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Abstract

The invention relates to an extracting method for stevioside in stevia rebaudiana. The extracting method comprises the following steps: extracting dry leaves of the stevia rebaudiana and remaining an extracting liquid; loading a sample of the extracting liquid to a separation column which is filled with Generik RP packing for separating a target component from impurity components in the extracting liquid; remaining the target component in the Generik RP packing into which the sample is loaded; subsequently, performing gradient elution on the target component by respectively adopting a pretreatment eluting liquid and a target eluting liquid, respectively obtaining a pretreated effluent liquid and a target effluent liquid, and remaining the target effluent liquid, wherein the pretreatment eluting liquid is a mixed liquid of an organic solvent and water, and the target eluting liquid is a mixed liquid of the organic solvent and water or the organic solvent; concentrating and drying the target effluent liquid to obtain the stevioside. The extracting method for the stevioside in the stevia rebaudiana has few steps and simple overall process, and is beneficial to application.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting steviol glycosides in stevia rebaudiana. Background technique [0002] Stevia rebaudiana, also known as stevia and sugar grass, belongs to Compositae. It contains 14 kinds of trace elements and 32 kinds of nutrients. It does not metabolize, accumulate, cause cancer and is non-toxic in the body. Its safety has been approved by the international FAO and WHO and the US FAD. organization, so it is both an excellent source of sugar and a good source of nutrition. [0003] Steviol glycoside is an active ingredient in stevia, which is a natural low-calorie high-intensity sweetener. It contains a variety of tetracyclic diterpene monomers with different components. These monomers all have the same aglycon-steviol, which can be divided into stevioside (Stevioside), rye Baudioside A (RebaudiosideA), B (RebaudiosideB), C (RebaudiosideC), D (Rebaud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256
Inventor 黄学英王吉
Owner SEPAX TECH
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