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A method for simultaneous determination of five aminoglycoside drug residues in food

A technology of aminoglycosides and residues, which is applied in the field of chromatographic detection, can solve the problems of interference in detection of other compounds, and achieve the effect of good sensitivity, easy operation and high sensitivity

Active Publication Date: 2018-05-01
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Another technical problem to be solved by the present invention is to provide an instrumental analysis method to solve the problem of interference in the detection of other compounds due to the addition of ion-pair reagents in existing methods

Method used

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  • A method for simultaneous determination of five aminoglycoside drug residues in food
  • A method for simultaneous determination of five aminoglycoside drug residues in food
  • A method for simultaneous determination of five aminoglycoside drug residues in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Detection of streptomycin, dihydrostreptomycin, kanamycin, spectinomycin, and gentamicin residues in fish meat

[0049] (1) Sample pretreatment

[0050] extract:

[0051] Weigh 3g of the crushed sample into a 50ml centrifuge tube, add 10ml of extract, homogenize, mix, and centrifuge, transfer the supernatant to another new 50ml centrifuge tube, add 10ml of n-hexane to the extract, Shake and mix for 15 minutes, centrifuge, discard the upper liquid, and take 10ml of the lower layer extract to purify through the column.

[0052] Purify:

[0053] SPE column purification, pre-column with 5ml of methanol and 5ml of water in advance, 10ml of extract solution plus liquid reservoir through the C18 column at a flow rate of less than or equal to 3ml / min, after the liquid flows out completely, rinse the column with 5ml of water, discard All the effluents were blown dry under positive pressure, and finally eluted with 3ml of methanol. The eluate was collected into a 10...

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Abstract

The invention discloses a measuring method for detecting residual quantities of streptomycin, dihydrostreptomycin, kanamycin, spectinomycin and neomycin in food simultaneously. The method comprises steps as follows: (1) extraction; (2) purification; (3) preparation of a matrix standard working solution; (4) measurement with LC-MS / MS (liquid chromatography-tandem mass spectrometry). The method has the advantages of simplicity in operation, accuracy, high sensitivity and good repeatability, can completely meet technical requirements for corresponding product security detection in China, the European Union, America and Japan and can provide powerful technical support for guaranteeing food security of people in China and sound development of export trades.

Description

technical field [0001] The present invention relates to a method for determining the residual amount of multiple aminoglycoside antibiotics at the same time, specifically five kinds of streptomycin, dihydrostreptomycin, kanamycin, spectinomycin and gentamicin The invention discloses a method for determining residues of aminoglycoside drugs, belonging to the technical field of chromatographic detection. Background technique [0002] Aminoglycoside antibiotics (aminoglycoside antibiotics, AGs) are glycosides formed from amino sugars and aminocyclitols. Since Waksman et al. discovered that Streptomyces produced streptomycin in 1944, the research and development of aminoglycoside antibiotics has passed more than half a century. Due to their stable properties, broad antibacterial spectrum, strong bactericidal power, good water solubility, rapid and complete excretion, and post-antibiotic effects on many pathogenic bacteria, these drugs are still clinically important anti-infecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/60
CPCG01N30/02G01N30/60G01N2030/027
Inventor 王凤美邱芳张鸿伟
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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