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An Analytical Method for Rapid Detection and Identification of Chemical Components in Glyphs

A technology of chemical composition and analysis method, applied in the field of Hook analysis, which can solve the problems of requiring standard products and cannot study the material basics of complex systems, and achieves low sample injection times, rapid and accurate analysis and detection, and simple operation. Effect

Active Publication Date: 2017-07-04
HUNAN AGRICULTURAL UNIV
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  • Description
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Problems solved by technology

After reviewing relevant literature, most of them can only detect and analyze a few chemicals, and require standard products, and cannot solve the material basis research of complex systems in terms of method and technology.
At present, there is no rapid detection and analysis method using liquid chromatography-quadrupole-time-of-flight mass spectrometry combined with self-built data packages

Method used

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  • An Analytical Method for Rapid Detection and Identification of Chemical Components in Glyphs
  • An Analytical Method for Rapid Detection and Identification of Chemical Components in Glyphs
  • An Analytical Method for Rapid Detection and Identification of Chemical Components in Glyphs

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Embodiment 1

[0029] 1. Instruments, reagents and materials

[0030]Instrument: Agilent 6530 liquid chromatography-quadrupole-time-of-flight mass spectrometer, analytical column is aODS-C18column (150mm×2.0mm I.D.; particle size 5μm).

[0031] Reagents: acetonitrile and formic acid are chromatographically pure. The water used was ultrapure water (deionized water).

[0032] Material: 0.22 μm aqueous syringe filter.

[0033] 2. Hook kiss alcohol extract

[0034] Add 80L of 95% ethanol to the extraction tank into which 10kg of hook kisses was placed, extract for 1.5h, filter the medicinal liquid with a 200-mesh filter screen, extract the medicinal residue once again in the same way, combine the two extracts, and place them in a concentration tank to concentrate To the relative density of about 1.2, it can be obtained after drying under reduced pressure.

[0035] 3. Detection

[0036] The liquid phase conditions of the analytical column are: mobile phase A is 0.1% formic acid water, B is a...

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Abstract

The invention discloses an analysis method for quickly detecting and identifying chemical components in gelsemium elegans. The analysis method includes detecting gelsemium elegans ethanol extract by the aid of liquid chromatography, quadrupole, time-of-flight mass spectrometers and finding accurate parent ion peaks in the gelsemium elegans ethanol extract by the aid of automatic MS / MS (mass spectrometry / mass spectrometry) processes; quickly automatically matching and analyzing gelsemium elegans data packets, finding possible molecular formulas and structural formulas of the chemical components in the gelsemium elegans ethanol extract and identifying the possible chemical components in the gelsemium elegans according to corresponding accurate two-level mass spectra. The method has the advantages that the chemical components in the gelsemium elegans can be quickly and accurately detected and identified, the method is easy to implement and high in analysis efficiency and throughput, and the like.

Description

technical field [0001] The invention relates to an analysis method for rapidly detecting and identifying chemical components in a hooked kiss, belonging to the technical field of hooked kiss analysis. Background technique [0002] Hook kiss, also known as heartbroken grass, kudzu, etc., is a world-famous highly poisonous plant. There are three species in the world: Gelsemium elegans Benth is mainly distributed in Fujian, Zhejiang, Yunnan, Guangxi, Hunan, Guizhou, Jiangxi and other southern regions and Southeast Asia; Gelsemium sempervirens (L.) J. st-Hil) and swamp jasmine (Gelsemium rankinii Small.), mainly distributed in North American countries. Chinese medicine has long recorded the hook kiss, as described in the Dictionary of Traditional Chinese Medicine: its roots, ridges, and leaves can be used as medicine, warm in nature, pungent, bitter, and highly toxic. It has the functions of dispelling wind and fighting toxins, dispersing knots, and relieving pain. It is mainl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/34
CPCG01N30/34G01N30/88
Inventor 刘兆颖刘艳纯孙志良
Owner HUNAN AGRICULTURAL UNIV
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