An Analytical Method for Rapid Detection and Identification of Chemical Components in Glyphs
A technology of chemical composition and analysis method, applied in the field of Hook analysis, which can solve the problems of requiring standard products and cannot study the material basics of complex systems, and achieves low sample injection times, rapid and accurate analysis and detection, and simple operation. Effect
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[0029] 1. Instruments, reagents and materials
[0030]Instrument: Agilent 6530 liquid chromatography-quadrupole-time-of-flight mass spectrometer, analytical column is aODS-C18column (150mm×2.0mm I.D.; particle size 5μm).
[0031] Reagents: acetonitrile and formic acid are chromatographically pure. The water used was ultrapure water (deionized water).
[0032] Material: 0.22 μm aqueous syringe filter.
[0033] 2. Hook kiss alcohol extract
[0034] Add 80L of 95% ethanol to the extraction tank into which 10kg of hook kisses was placed, extract for 1.5h, filter the medicinal liquid with a 200-mesh filter screen, extract the medicinal residue once again in the same way, combine the two extracts, and place them in a concentration tank to concentrate To the relative density of about 1.2, it can be obtained after drying under reduced pressure.
[0035] 3. Detection
[0036] The liquid phase conditions of the analytical column are: mobile phase A is 0.1% formic acid water, B is a...
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