Analysis method for quickly detecting and identifying chemical components in gelsemium elegans
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A technology of chemical composition and analysis method, which is applied in the field of kiss analysis, can solve the problems of needing standard products and not being able to check the basic research of complex systems, and achieves less number of injections, fast and accurate analysis and detection, and simple operation Effect
Active Publication Date: 2016-05-11
HUNAN AGRICULTURAL UNIV
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Problems solved by technology
After reviewing relevant literature, most of them can only detect and analyze a few chemicals, and require standard products, and cannot solve the material basis research of complex systems in terms of method and technology.
At present, there is no rapid detection and analysis method using liquid chromatography-quadrupole-time-of-flight mass spectrometry combined with self-built data packages
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Embodiment 1
[0029] 1. Instruments, reagents and materials
[0030]Instrument: Agilent6530 liquid chromatography-quadrupole-time-of-flight mass spectrometer, the analytical column is aODS-C18column (150mm×2.0mmI.D.; particlesize5μm).
[0031] Reagents: Acetonitrile and formic acid are chromatographically pure. The water used was ultrapure water (deionized water).
[0032] Material: 0.22 μm aqueous syringe filter.
[0033] 2. Alcoholic extract of Gelkinus
[0034] Add 80L of 95% ethanol to the extraction tank with 10kg of gills, extract for 1.5 hours, filter the medicinal liquid with a 200-mesh filter, extract the medicinal residue once more in the same way, combine the two extractions, and concentrate in the concentration tank When the relative density is about 1.2, it can be obtained after drying under reduced pressure.
[0035] 3. Detection
[0036] The liquid phase condition of described analysis column is: mobile phase A is 0.1% formic acid water, B is acetonitrile; Gradient eluti...
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Abstract
The invention discloses an analysis method for quickly detecting and identifying chemical components in gelsemium elegans. The analysis method includes detecting gelsemium elegans ethanol extract by the aid of liquid chromatography, quadrupole, time-of-flight mass spectrometers and finding accurate parent ion peaks in the gelsemium elegans ethanol extract by the aid of automatic MS / MS (mass spectrometry / mass spectrometry) processes; quickly automatically matching and analyzing gelsemium elegans data packets, finding possible molecular formulas and structural formulas of the chemical components in the gelsemium elegans ethanol extract and identifying the possible chemical components in the gelsemium elegans according to corresponding accurate two-level mass spectra. The method has the advantages that the chemical components in the gelsemium elegans can be quickly and accurately detected and identified, the method is easy to implement and high in analysis efficiency and throughput, and the like.
Description
technical field [0001] The invention relates to an analysis method for rapidly detecting and identifying chemical components in goblins, belonging to the technical field of goblin analysis. Background technique [0002] Ginkgo, also known as heartbroken grass, kudzu, etc., is a world-renowned highly poisonous plant. There are three species in the world, namely: Gelsemium elegans Benth is mainly distributed in southern China, such as Fujian, Zhejiang, Yunnan, Guangxi, Hunan, Guizhou, Jiangxi, and Southeast Asia; Gelsemium sempervirens (L. ) J.st-Hil) and marsh jasmine (Gelsemium rankinii Small.), mainly distributed in North American countries. Chinese medicine has long recorded the hook kiss, as described in the "Dictionary of Chinese Medicine": its roots, ridges, and leaves can be used as medicine, warm in nature, pungent, bitter in taste, and highly poisonous. It has the functions of expelling wind and attacking poison, dispelling stagnation, and relieving pain. It is mai...
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