A Bacteria Automatic Sorting and Marking Device Based on Immunization Method

Abstract

The invention discloses an automatic bacteria sorting and marking device based on an immune method, comprising a substrate; the surface of the substrate is respectively provided with a magnetic bead fluid tank and a fluorescent material tank; the magnetic bead fluid tank is connected with a first liquid pump by a duct; the first liquid pump is connected with a first culture tank by a duct; a first injection hole is formed in the top of the first culture tank; the fluorescent material tank is connected with a second liquid pump by a duct. According to the automatic bacteria sorting and marking device based on the immune method, a controller is used for respectively controlling the amount of culture bacteria injected into a third culture tank by the first culture tank and a second culture tank, so that quantitative and accurate culture is realized; magnetic bead-purpose complex bacteria is absorbed by electromagnet, so that a good effect is achieved; the third culture tank is directly connected with a waste liquid tank and a cleaning pipe, so that cleaning efficiency and purifying efficiency are better improved, the purifying time is short, and the economic benefit is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacterial automatic sorting and marking device based on an immune method. Background technique [0002] With the continuous development of life sciences, the basic research of bacterial biology and immunology, as well as the diagnosis and treatment of clinical diseases require the isolation and purification of bacteria from mixed samples containing different bacteria. Existing bacterial separation techniques mainly include Ficoll density gradient centrifugation, centrifugal elution, two-phase separation, etc. These methods are based on the characteristics of bacterial size, density, surface charge and other physical properties to separate, and it is difficult to obtain ideal purity and Recovery rate. [0003] Cell sorting based on magnetic beads is a cell separation technology that has developed rapidly in recent years. First, antibody molecules are labeled on magnetic beads to ma...

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Problems solved by technology

However, the operation in the cultivation process is cumbersome, quantitative and accurate cultivation cannot be achieved, the purification process is long, and the purification efficiency is low. Therefore, we propose an automatic bacterial sorting and marking device based on immune methods

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