Cultivating method for improving production capacity of micromonospora

A Micromonospora production capacity technology, applied in the field of cultivation to improve Micromonospora production capacity, can solve the problems of Micromonospora easy to decline, and achieve the effect of increasing production and stability

Inactive Publication Date: 2018-09-04
LIVZON GROUP FUZHOU FUXING PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: provide a kind of culture method that can improve Micromonospora production capacity, solve the problem that Micromonospora is easy to decay in conventional method

Method used

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  • Cultivating method for improving production capacity of micromonospora
  • Cultivating method for improving production capacity of micromonospora
  • Cultivating method for improving production capacity of micromonospora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A culture method for improving Micromonospora production capacity, comprising the following steps:

[0058] Step 1: Inoculate the single colony of Micromonospora obtained by isolation and purification culture into the slant medium, cultivate for 11 days, and obtain the first colony; the slant medium is configured by the following formula:

[0059] Soluble starch 7.5g / L; sodium chloride 0.5g / L; potassium dihydrogen phosphate 0.3g / L; sodium nitrate 1.0g / L; calcium carbonate 1.0g / L; agar powder 17g / L;

[0060] The separation and purification culture method is as follows: inoculate Micromonospora species into the separation and purification medium, and cultivate for 13 days to obtain a single Micromonospora colony with a diameter of 4-6 mm and a height of 3-4 mm; the separation and purification culture The base is configured from the following recipe:

[0061] Soluble starch 7.5g / L; sodium chloride 0.5g / L; potassium dihydrogen phosphate 0.3g / L; sodium nitrate 1.0g / L; calci...

Embodiment 2

[0073] A culture method for improving Micromonospora production capacity, comprising the following steps:

[0074] Step 1: Inoculate the single colony of Micromonospora obtained by isolation and purification culture into the slant medium, cultivate for 11 days, and obtain the first colony; the slant medium is configured by the following formula:

[0075] Soluble starch 7.0g / L; sodium chloride 0.3g / L; potassium dihydrogen phosphate 0.2g / L; sodium nitrate 0.8g / L; calcium carbonate 0.8g / L; agar powder 15g / L;

[0076] The separation and purification culture method is as follows: inoculate Micromonospora species into the separation and purification medium, and cultivate for 13 days to obtain a single Micromonospora colony with a diameter of 4-6 mm and a height of 3-4 mm; the separation and purification culture The base is configured from the following recipe:

[0077] Soluble starch 7.0g / L; sodium chloride 0.3g / L; potassium dihydrogen phosphate 0.2g / L; sodium nitrate 0.8g / L; calci...

Embodiment 3

[0086] A culture method for improving Micromonospora production capacity, comprising the following steps:

[0087] Step 1: Inoculate the single colony of Micromonospora obtained by isolation and purification culture into the slant medium, cultivate for 11 days, and obtain the first colony; the slant medium is configured by the following formula:

[0088] Soluble starch 8.0g / L; sodium chloride 0.7g / L; potassium dihydrogen phosphate 0.4g / L; sodium nitrate 1.2g / L; calcium carbonate 1.2g / L; agar powder 19g / L;

[0089] The separation and purification culture method is as follows: inoculate Micromonospora species into the separation and purification medium, and cultivate for 13 days to obtain a single Micromonospora colony with a diameter of 4-6 mm and a height of 3-4 mm; the separation and purification culture The base is configured from the following recipe:

[0090] Soluble starch 8.0g / L; sodium chloride 0.7g / L; potassium dihydrogen phosphate 0.4g / L; sodium nitrate 1.2g / L; calci...

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Abstract

The invention belongs to the field of industrial microorganisms, and particularly relates to a cultivating method for improving production capacity of micromonospora. The method comprises the following steps: successively performing slant culture, seed culture and fermentation culture on a micromonospora colony; inoculating the micromonospora colony obtained by separation and purification cultureon a slant culture medium, cultivating for 11 d to obtain a first bacterial colony; inoculating the first bacterial colony prepared by the culture of the slant culture medium on a seed culture medium,cultivating for 40 h to obtain first bacteria solution; and inoculating the first bacteria solution prepared by the culture of the seed culture medium on a fermentation culture medium, cultivating for 120 h to obtain second bacteria solution, wherein the second bacteria solution is the micromonospora with the high production capacity. Through the improvement of a culture medium formula of the slant culture, the seed culture and the fermentation culture and culture time, so a verification flask shaking titer of the prepared second bacteria solution can be improved by 20-30%.

Description

technical field [0001] The invention belongs to the field of industrial microorganisms, in particular to a cultivation method for improving the production capacity of Micromonospora. Background technique [0002] Micromonospora has been used in the field of industrial microorganisms for a long time and is the production strain of gentamicin. During the production process of Micromonospora, it was found that Micromonospora cultivated by conventional methods tended to decline, which affected the fermentation level and resulted in unstable gentamicin production. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a culture method capable of improving the production capacity of Micromonospora, and to solve the problem that Micromonospora is easy to decline in conventional methods. [0004] In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/29
CPCC12N1/20
Inventor 黄凤珠周兵兵王为民龚振宝吴美蓉巫秋萍
Owner LIVZON GROUP FUZHOU FUXING PHARMACEUTICAL CO LTD
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