Solid culture method for entomopathogenic nematodes

A technology of entomopathogenic nematodes and solid culture, applied in bacteria, animal husbandry, etc., can solve problems such as threats to human health, unsatisfactory effects, and impact on crop quality

Active Publication Date: 2016-08-03
ZHEJIANG LYUDUN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the control of underground pests is still dominated by chemical insecticides, but the effect is not ideal, and it is easy to cause environmental pollution, threaten human health and other negative effects
Controlling

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] 1. Medium formula:

[0031] Strain culture medium formula: peptone 1g, sodium chloride 0.5g, yeast 0.5g, water 980ml.

[0032] 2. Activation of nematode symbiotic bacterial strains

[0033] Inoculate the nematode symbiotic bacteria into the strain medium for activation, and culture at 25°C for 1 to 2 days

[0034] 3. Preparation of symbiotic bacteria liquid

[0035] Insert the activated nematode symbiotic bacteria (preferably a single colony) into 500 ml of shake flask culture solution, and shake and culture at 160 rpm for 1 to 2 days at 25±1° C. to obtain a symbiotic bacteria liquid.

[0036] 4. Preparation of solid medium

[0037] Prepare the solid medium according to the solid medium formula, and the solid medium formula is as follows: soybean powder 15%, flour 10%, egg yolk powder 1%, peptone 1%, vegetable oil 1%, yeast 1%, sponge 10%, water 61%.

[0038] 5. Solid culture container, capacity and sterilization

[0039] Stainless steel culture box: length 50cm, w...

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PUM

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Abstract

The invention discloses a solid culture method for entomopathogenic nematodes. The method is characterized by comprising the specific steps: (a) preparing an inoculum culture medium, activating symbiotic bacteria of the entomopathogenic nematodes, and inoculating the symbiotic bacteria to the inoculum culture medium; (b) inoculating a bacterial solution of the symbiotic bacteria to a solid culture medium; (c) carrying out culture for 3 to 5 days in the solid culture medium, and then, inoculating an entomopathogenic nematode suspension to the solid culture medium; (d) carrying out dark static culture on the nematodes for three weeks, thereby harvesting the nematodes. According to the method, the problem that bioculture methods cannot achieve the large-scale production of the entomopathogenic nematodes is solved, the production cost of the solid culture method is obviously lower than that of a liquid culture method, and the pathogenicity of the nematodes produced by the solid culture method is apparently higher than that of the nematodes produced by the liquid culture method. The market demand is increased, and the solid culture method has better prospects of development and has an all-important significance in the large-scale production of the entomopathogenic nematodes and the biological control on soil insects.

Description

【Technical field】 [0001] The invention relates to the technical field of biological propagation and cultivation, in particular to the technical field of solid culture methods for entomopathogenic nematodes. 【Background technique】 [0002] Subterranean pests refer to omnivorous insects that live in the soil and harm the underground parts of plants, seeds, seedlings or main stems near the soil surface during their whole life or at a certain stage of their life. Subterranean pests are the great enemies of crops. They harm seeds, young shoots, and rhizomes, resulting in lack of seedlings and even destruction of seeds, resulting in reduced crop yields, especially grubs, mole crickets, cutworms, and needleworms. [0003] At present, chemical insecticides are still the main method for underground pest control, but the effect is not ideal, and it is easy to cause environmental pollution and threaten human health and other negative effects. Controlling underground pests through high...

Claims

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Application Information

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IPC IPC(8): A01K67/033C12N1/20
CPCA01K67/033C12N1/20
Inventor 蒋千盛锡良
Owner ZHEJIANG LYUDUN BIOTECH CO LTD
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