Human adenovirus antigen epitope chimeric protein as well as preparation and application thereof

A technology of antigenic epitopes and chimeric proteins, which can be applied to antiviral immunoglobulins, viruses, viral peptides, etc., and can solve problems such as false negatives

Active Publication Date: 2016-08-03
中国人民解放军东部战区疾病预防控制中心
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  • Description
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AI Technical Summary

Problems solved by technology

However, at present, one antigen can only detect one type of adenovirus antibody, so when detecting HAdV antibodies in serum, it is necessary to use multiple antigens to improve the detection rate of antibodies, confirm the infection type, increase the detection cost, and there are still some The false negative probability of

Method used

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  • Human adenovirus antigen epitope chimeric protein as well as preparation and application thereof
  • Human adenovirus antigen epitope chimeric protein as well as preparation and application thereof
  • Human adenovirus antigen epitope chimeric protein as well as preparation and application thereof

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Embodiment Construction

[0151] Detailed description of the embodiments of the present invention:

[0152] Analysis, gene synthesis and expression of antigen epitope of adenovirus hexon protein

[0153] The amino acid sequence of adenovirus type 3, 7, 11, 14 and 55 type hexon proteins were analyzed by computer, and the protein fragments with strong antigenicity were screened out. Four protein fragments were screened for adenovirus type 3, four protein fragments were screened for adenovirus type 7, two protein fragments for adenovirus type 11, four protein fragments for adenovirus type 14, and two for adenovirus type 55 Protein fragments. The protein fragments are connected by two glycines and one serine to form a multiple epitope chimeric protein in series. Use the codons preferred by prokaryotes to translate the chimeric protein into the corresponding nucleotide sequence. The full-length gene sequence of the chimeric protein was chemically synthesized and cloned into the NdeI / EcoRI site of plasmid pET-...

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Abstract

The invention discloses human adenovirus antigen epitope chimeric protein as well as preparation and application thereof, and relates to fields of gene engineering techniques, vaccines and diagnostic reagents. The amino acid sequences of hexon protein of type 3, type 7, type 11, type 14 and type 55 of adenovirus are analyzed through computer analysis, protein fragments with good antigenicity can be screened respectively, the fragments are connected by using two glycine and one serine, then chimeric protein with multiple antigen fragments in serial connection can be formed, pronucleus preferred codons are selected and interpreted into corresponding nucleotide sequences, and full-length genes can be synthesized in a chemical manner. The chimeric protein can be obtained through expression purification by using a gene engineering technique, and the chimeric protein comprises 363 amino acids in whole length. The expressed chimeric protein can be applied to vaccine research, HAdV antibody or antigen detection and the like, and is related to fields such as gene engineering techniques, vaccines and diagnostic reagents.

Description

Technical field [0001] The human adenovirus epitope chimeric protein and its preparation and application of the present invention relate to the fields of genetic engineering technology, vaccines and diagnostic reagents. The present invention connects human adenovirus (HAdV) type 3, 7, 11, 14 and 55 specific epitope protein fragments in series, and uses genetic engineering technology to prepare strong antigen epitopes containing five types of adenovirus The chimeric protein. Through computer analysis, the protein fragments containing strong epitopes were selected from the five types of adenovirus hexon proteins, and the protein fragments were connected by two glycines and one serine to form a multiple epitope chimeric protein. . The gene sequence of the chimeric protein is chemically synthesized, and the chimeric protein is expressed by genetic engineering technology. The expressed chimeric protein can be used for HAdV antibody or antigen detection, and vaccine development. B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K1/36C07K1/30C07K1/18C07K16/08C12N15/62C12N15/70A61K39/295A61K39/235A61P31/14G01N33/569
CPCA61K39/00C07K14/005C07K16/081C07K2319/00C12N15/70C12N2710/10022C12N2800/101C12N2800/22G01N33/56983G01N2333/075
Inventor 李越希王新国李佳萌李素芹陈红霞陈晨李素梅齐勇潘英徐亦非
Owner 中国人民解放军东部战区疾病预防控制中心
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