A method for identifying the function of maize male fertility gene

Abstract

The invention discloses a method for identifying corn male fertility gene functions. The method provided by the invention is manly characterized in that through transgenosis, group preparation and development of molecular markers tightly linked with the target property, individuals of the genes to be tested expressing the normal fertility function under the condition of the corn recessive homozygous mutation background are obtained and identified; meanwhile, the phenotype is verified, so that the function of the genes to be tested is verified. Through the group preparation, the problems that the corn material capable of being used as a genetic transformation receptor is in the male fertile phenotype, the function of the gene for controlling the corn male fertility cannot be directly identified through transgenosis are solved; the molecular marker tightly linked with the male fertility is ingeniously used, so that the difficult problem that after the transgenic fragments containing the normal target genes are introduced into the corn male sterile materials, the target gene homozygous mutation background cannot be identified and distinguished by transgenic positive plants in the separation later generations is solved.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for identifying the function of maize male fertility genes. Background technique [0002] Male sterility is a common phenomenon in plants, which refers to the phenomenon that the male reproductive organs cannot produce normal pollen, but the female reproductive organs develop normally, can receive normal pollen, fertilize and set fruit, and can pass the sterility to offspring. Male sterility is mainly manifested in deformed or degenerated stamens, or thin, shrunken anthers, and lack of pollen or pollen empty and lifeless. Male sterility in maize includes plasmonuclear interaction male sterility and nuclear gene control male sterility. During the development of male reproductive organs in maize, mutations in any nuclear male fertility gene may lead to male sterility. This kind of male sterility can be divided into dominant sterility and recessive sterility, and most of them ar...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problem that the maize material that can be used as a genetic transformation recipient is a male-fertile phenotype, and the function of the gene controlling maize male fertility cannot be directly identified through the transgene, and provides a method for verifying the function of the maize male-fertility gene

This method also solves the problem that the homozygous mutation background individuals of the target gene cannot be identified in the isolated offspring after the transgene fragment containing the normal target gene is introduced into the maize male sterile material

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