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Banana-fruit-particle/amylosynthease-gene-promoter-combined key response element and screening method thereof

A technology of starch synthase and response element, which is applied in the key response element and its screening field of banana fruit granule binding starch synthase gene promoter, which can solve the problems of incompletely clear expression regulation mechanism, hard core, poor waxiness, etc.

Active Publication Date: 2016-09-21
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

[0003] At present, the high-quality banana fruit rate in my country is less than 30%, and most banana fruits have quality problems such as hard core, poor waxiness, low sugar content, and unstable resistant starch content during the post-ripening process.
However, the expression regulation mechanism of MaGBSSI-3 is still not completely clear, and it may be regulated by multiple levels and multiple factors, and further research is needed to confirm

Method used

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  • Banana-fruit-particle/amylosynthease-gene-promoter-combined key response element and screening method thereof
  • Banana-fruit-particle/amylosynthease-gene-promoter-combined key response element and screening method thereof
  • Banana-fruit-particle/amylosynthease-gene-promoter-combined key response element and screening method thereof

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[0023] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0024] 1. Deletion of the 5′ end of the MaGBSSI-3 gene promoter

[0025] In order to further study the obtained promoter response element of MaGBSSI-3, through the bioinformatics analysis of the promoter sequence, the promoter fragment was deleted with different lengths at the 5' end and a transient expression vector was constructed. Using the full length of the cloned promoter as a template, design the 5′-end deletion primer sequence (the 3′-...

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Abstract

The invention provides a banana-fruit-particle / amylosynthease-gene-promoter-combined key response element and a screening method thereof. The key response element is a DNA segment obtained by carrying out PCR (polymerase chain reaction) amplification on the template Brazilian banana genome DNA by using primers of which the nucleotide sequences are disclosed as SEQ ID NO.7 and SEQ ID NO.8. Banana MaGBSSI-3 promoter deleted segments with different lengths are designed for screening so as to obtain the key response element of the MaGBSSI-3 gene promoter, thereby providing direction in the research of demands for different contents of amyloses in food, medical treatment, industry and other professions, and providing references for research of GBSS gene of other plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a key response element of banana fruit granules bound to a starch synthase gene promoter and a screening method thereof. Background technique [0002] Banana is an important economic crop in tropical and subtropical regions, and it is the second largest fruit in the world after citrus. As the main component of banana fruit, starch has prompted it to be identified by the Food and Agriculture Organization of the United Nations (FAO) as the fourth largest food crop after rice, wheat, and corn, and is the ration of more than 400 million people (Englberger et al. ,2006). However, with the rapid development of the banana industry and the rapid improvement of people's living standards, the requirements for the quality of banana fruit are also getting higher and higher. Applying modern biotechnology to banana quality improvement can solve some problems that are diffi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/11C12Q1/68
CPCC12N9/1051C12Q1/6895C12Y204/01021
Inventor 苗红霞孙佩光金志强徐碧玉刘菊华张建斌贾彩红王卓王静毅
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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