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Polygala fallax Hemsl in-vitro rapid propagation and seedling breeding method

A technique for rapid propagation of yellow-flowered water lotus and seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of stability and repeatability limitations, achieve good disinfection effect, accelerate reproduction speed, Stable and repeatable effect

Inactive Publication Date: 2016-10-12
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology

However, this invention has certain limitations in terms of stability and repeatability

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Embodiment Construction

[0030] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments, which are only used to explain the present invention and do not limit the protection scope of the present invention.

[0031] EXAMPLE Taking the required steps and specific parameters for the establishment of the in vitro rapid propagation system of Lily chrysanthemum chrysanthemum as the object, the following experiments were carried out

[0032] 1 Materials and methods

[0033] 1.1 Test material

[0034] The test materials were the branches with tender stems of the current year, which were taken from Qingliu County, Sanming City, Fujian Province.

[0035] 1.2 Test method

[0036] 1.2.1 Material pretreatment

[0037] The explants were rinsed in detergent water for 3-5 min, and then rinsed with running water for 30 min. During the cleaning process, the branches that are too long or too large...

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Abstract

The invention discloses a Polygala fallax Hemsl in-vitro rapid propagation and seedling breeding method. The method includes: taking Polygala fallax Hemsl tender stem as the explant, performing disinfection, then inoculating the explant to an initial medium of MS+6-BA 2.0mg / L+NAA 0.05mg / L to induce adventitious buds, then inoculating the adventitious buds to a proliferation and rooting medium attached with exogenous hormones of different types and concentrations to conduct proliferation and rooting culture, and transplanting the rooting test-tube plantlets into a seedling hardening matrix to perform seedling hardening. The Polygala fallax Hemsl in-vitro rapid propagation system established by the invention has the advantages of convenient material taking, good stability and high repeatability, and provides a method for realizing rapid propagation of Polygala fallax Hemsl high-quality germplasms and solving the current situation that the lack of wild resources causes demand exceeding supply in production.

Description

technical field [0001] The invention relates to the technical field of in vitro rapid propagation of chrysanthemum chrysanthemum, in particular to a method for in vitro rapid propagation of chrysanthemum chrysalis. Background technique [0002] Yellow-flowered water lotus ( Polygala fallax Hemsl.) is also known as Huanghua ginseng, Huanghuadiaoshuilian, Guanyin string, Huanghua Dayuanzhi, etc. It belongs to the genus Polygalaceae (Polygalaceae) ( Polygala ) plants[1]. Mainly distributed in my country's Fujian, Hunan, Guangxi and other places [2-4]. It is a relatively rare Chinese medicinal material. The whole herb is used as medicine, and it is sweet in nature and slightly bitter in taste. It has the functions of nourishing qi and blood, invigorating the spleen and promoting dampness, promoting blood circulation and regulating menstruation. It is a folk drug commonly used by ethnic minorities such as Yao, Miao, and Zhuang [5]. As a precious medicinal material, chrysant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 辛培尧辛亚龙唐军荣李斌费希同何承忠韩国伟
Owner SOUTHWEST FORESTRY UNIVERSITY
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