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A method for biotransformation and degradation of astragaloside IV to prepare cycloastragenol

A technology of astragaloside IV and cycloastragenol, which is applied in the field of medicine and chemical industry, can solve the problems that cycloastragenol has not been reported, and achieve the effects of solving raw material restrictions, high conversion specificity, and low cost

Active Publication Date: 2019-11-08
安徽中鸣科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The method for preparing cycloastragenol by microbial fermentation technology has not yet been reported

Method used

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  • A method for biotransformation and degradation of astragaloside IV to prepare cycloastragenol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The present embodiment prepares cycloastragenol according to the following steps:

[0028] (1) Seed cultivation

[0029] Prepare PDB medium in the Erlenmeyer flask, then inoculate the strain Absidia sp.CGMCC 3.2834, and culture it for 3 days under the conditions of shaker temperature 30°C and shaker speed 180r / min to obtain the primary medium for seeds ;

[0030] (2) Transformation culture

[0031] Insert the primary seed medium obtained in step (1) into 150 mL of improved PDB medium at an inoculum size of 10%, and cultivate it for 3 days at a shaker temperature of 30°C and a shaker speed of 180r / min, and then add 50mg of astragaloside IV substrate (astragaloside IV containing 34.5 mg), continued to cultivate for 6 days to obtain fermentation broth;

[0032] Mix the fermented liquid obtained in step (2) with the extractant ethyl acetate in equal volumes, extract three times at room temperature for 10 min each time, and concentrate the obtained extract to dryness in v...

Embodiment 2

[0038] The present embodiment prepares cycloastragenol according to the following steps:

[0039] (1) Seed cultivation

[0040] Prepare PDB medium in Erlenmeyer flask, then inoculate the strain Absidia sp.CGMCC 3.2834 for fermentation, and cultivate for 3 days under the conditions of shaker temperature 30°C and shaker speed 180r / min to obtain the primary culture of seeds base;

[0041] (2) Transformation culture

[0042] Insert the primary seed medium obtained in step (1) into 150mL improved PDB medium according to the inoculum size of 5%, and cultivate it for 3 days under the conditions of a shaker temperature of 30°C and a shaker speed of 180r / min, and then add 50mg of astragaloside IV substrate (astragaloside IV containing 34.5 mg), continued to cultivate for 6 days to obtain fermentation broth;

[0043] (3) Purification

[0044] Mix the fermented liquid obtained in step (2) with the extractant ethyl acetate in equal volumes, extract three times at room temperature for ...

Embodiment 3

[0049] The present embodiment prepares cycloastragenol according to the following steps:

[0050] (1) Seed cultivation

[0051] Prepare PDB medium in the Erlenmeyer flask, then inoculate the strain Absidia sp.CGMCC 3.2834, and culture it for 3 days under the conditions of shaker temperature 30°C and shaker speed 180r / min to obtain the primary medium for seeds ;

[0052] (2) Transformation culture

[0053] Insert the seed primary medium obtained in step (1) into 150mL common PDB medium according to the inoculum amount of 10%, and cultivate it for 3 days under the conditions of the shaker temperature of 30°C and the shaker speed of 180r / min, and then add 50mg of astragaloside IV substrate (astragaloside IV containing 34.5 mg), continued to cultivate for 6 days to obtain fermentation broth;

[0054] (3) Purification

[0055] Mix the fermented liquid obtained in step (2) with the extractant ethyl acetate in equal volumes, extract three times at room temperature for 10 min each...

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Abstract

The invention discloses a method for preparing cycloastragenol by means of biological conversion and degradation of astragaloside. The method is characterized in that astragaloside serves as a substrate, and cycloastragenol is prepared by means of fermentation of the strain of Absidia sp.CGMCC 3.2834. According to the method for preparing cycloastragenol by means of biological conversion and degradation of astragaloside, a microbiologic fermentation technology is adopted for degrading astragaloside to obtain high-purity cycloastragenol, the technology is simple and easy to carry out, the cost is low, the conversion rate of cycloastragenol reaches 60% or above, the purity reaches up to 95% or above, and the conversion specificity is high; the whole technological process is conducted at normal temperature and atmospheric pressure, the required equipment conditions are low, and the method is suitable for mass production.

Description

technical field [0001] The invention discloses a method for biotransforming and degrading astragaloside IV to prepare cycloastragenol, which belongs to the field of medicine and chemical industry. Background technique [0002] Astragalus is the dried root of the leguminous plant Astragalus mongolica or Astragalus membranaceus. It was first recorded in "Shen Nong's Materia Medica", and has a medicinal history of more than 400 years. It is still one of the most commonly used drugs in clinical treatment of traditional Chinese medicine. Saponins are the most important active ingredients in plants of the genus Astragalus, and the saponins contained therein are mainly cycloartane-type tetracyclic triterpenoid saponins structurally. Cycloastragenol is the aglycone moiety of astragaloside with the molecular formula C 30 h 50 o 5 , The relative molecular weight is 490.71. Its content in the plant is very small, accounting for about 0.1% of the dry weight. It is the main product ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/65
CPCC12P33/20
Inventor 陈彦王亚王力鸣蔡正楠杨维维
Owner 安徽中鸣科技有限公司
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