Kit for amplifying Her2 (Human epidermal growth factor receptor-2) gene in human peripheral blood circulating tumor DNA (Deoxyribonucleic Acid)

A human peripheral blood and gene amplification technology, applied in the biological field, can solve the problems of unsatisfactory detection accuracy, repeatability and integrity of breast cancer HER2, expensive, high technical requirements of FISH, and achieve reliable detection results and easy operation Simple, sensitivity-enhancing effects

Inactive Publication Date: 2016-10-12
BEIJING ABACE BIOTECH
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most hospitals in China have carried out HER2IHC detection. This method is simple, easy, and cheap, but it is easily affected by many factors.
Foreign probe kits and targeted drugs (Herceptin)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for amplifying Her2 (Human epidermal growth factor receptor-2) gene in human peripheral blood circulating tumor DNA (Deoxyribonucleic Acid)
  • Kit for amplifying Her2 (Human epidermal growth factor receptor-2) gene in human peripheral blood circulating tumor DNA (Deoxyribonucleic Acid)
  • Kit for amplifying Her2 (Human epidermal growth factor receptor-2) gene in human peripheral blood circulating tumor DNA (Deoxyribonucleic Acid)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Materials: Plasma samples to be tested, positive quality control products, and negative quality control products. The positive quality control product is genomic DNA of a breast cancer patient, and the negative quality control product is normal human genome DNA.

[0036]Primers and probes used: Her2 gene target site primers: SEQ ID NO: 1 and SEQ ID NO: 2; Her2 gene-specific hydrolysis probe SEQ ID NO: 3; primers for internal reference genes SEQ ID NO: 4 and SEQ ID NO: 5. The hydrolysis probe of the internal reference gene is SEQ ID NO: 14, wherein the primers and probes for the target site of the Her2 gene are designed and screened according to the ATM region SEQ ID NO: 19 of the ataxia telangiectasia mutation of the Her2 gene. The purity of all primers and probes should reach electrophoresis grade (PAGE) or HPLC grade, free of impurities. Provide the quality inspection certificate of the synthetic product issued by the synthesis institution, such as the PAGE electroph...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a kit for amplifying a Her2 (Human epidermal growth factor receptor-2) gene in human peripheral blood circulating tumor DNA (Deoxyribonucleic Acid). By adopting component setting of the kit and a result reading scheme, a detection process is simple and convenient and the operability is strong. A detection result is reliable and has good specificity; meanwhile, a real-time fluorescence PCR (Polymerase Chain Reaction) technology is used for detecting an amplification level of the Her2 gene in a breast cancer tissue specimen through calculating a deltaCt value; the kit can be used for a plurality of research fields including breast cancer auxiliary diagnosis carried out through detection of ctDNA of the blood, clinical application, prognosis and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a detection kit for Her2 gene amplification in human peripheral blood circulating tumor DNA. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, and its incidence is increasing year by year. In European and American countries, breast cancer accounts for 25%-30% of female malignant tumors. Statistics at the end of the 20th century show that about 1.3 million people in the world are diagnosed with breast cancer every year, and 400,000 people die from the disease. In many big cities in my country, the incidence of breast cancer has risen to the first or second place in female malignant tumors, and the death rate has taken the fourth or fifth place, becoming the biggest threat to women's health. [0003] Epidermal growth factor receptor-2 (Epidermal growth factor receptor-2, HER2), a receptor located on the surface of breast and b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C12Q2545/101C12Q2531/113C12Q2563/107
Inventor 章文羿李晶崔航黄清瑞
Owner BEIJING ABACE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap