Immobilized microbial system as well as preparation method thereof and water toxicity detection method

A microbial carrier and microbial technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, fixed on or in inorganic carriers, etc., can solve the problems of poor detection effect and low activity, and achieve good detection effect. The effect of high activity and simple detection method

Active Publication Date: 2016-10-26
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an improved way to make certain types of materials more effective at carrying germs or other organism(s). These techniques involve fixing tiny particles called bacteria onto these carriers with adhesive agents like glue or silicone oil. By doing this they become attached themselves into spaces between them instead of being tied up together. They also have a 3 - dimensional framework made from solidified plastic (plastisolymer) resin, allowing it to transport liquids through its holes while still keeping their original shape when placed inside another container filled with air. Overall, this new technique simplifies the production processes and improves efficiency over existing methods used today.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing effective methods for identifying harmful agents (pesticide) in marine ecosystems without causing any negative effects upon humans who live near them due to these harshness aspects like physical force impact and high levels of pesrotoxicoids found naturally within ocean waters. Previous attempts were made based solely on static measurements only, making it hard to identify threat sources quickly enough during global environmental assessment campaigns. Additionally, previous approaches had limitations in terms of sensitivity and response times, leading to less accurate data collection and potential overall, current methods lack efficiency and accuracy.

Method used

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  • Immobilized microbial system as well as preparation method thereof and water toxicity detection method
  • Immobilized microbial system as well as preparation method thereof and water toxicity detection method
  • Immobilized microbial system as well as preparation method thereof and water toxicity detection method

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preparation example Construction

[0064] The invention provides a preparation method of a fixed microbial system, comprising:

[0065] Attaching microorganisms to the surface of the microbial carrier to obtain an immobilized microbial system; or

[0066] Cultivate microorganisms in situ on the surface of the microbial carrier to obtain a fixed microbial system;

[0067] The microbial carrier is prepared by carbonizing plant tissue.

[0068] In the present invention, the microorganisms and microbial carriers are consistent with the microorganisms and microbial carriers described in the above technical solution, and will not be repeated here.

[0069] The present invention has no special limitation on the method for attaching microorganisms to the surface of the microbial carrier, and those skilled in the art can transfer the microorganisms to the surface of the microbial carrier according to actual operating conditions.

[0070] In the present invention, the temperature for culturing microorganisms in situ is...

Embodiment 1

[0110] Wash the loofah and cut it to the desired size. Put it into a reaction kettle, carry out carbonization at 180°C for 6 hours, put the obtained carbonized product into ethanol to remove organic residues; then rinse with secondary water and freeze-dry under the condition of 0.08mpar for later use to obtain the microbial carrier.

[0111] The microbial carrier prepared in Example 1 is characterized by SEM, and the detection results are as follows: figure 1 as shown, figure 1 It is the SEM image of the microbial carrier prepared in Example 1 of the present invention.

Embodiment 2

[0113] The microbial carrier was prepared according to the method described in Example 1, the difference from Example 1 was that scallions were used instead of loofah pulp.

[0114] Carry out SEM characterization to the microbial carrier that embodiment 2 prepares, detection result is as follows figure 2 as shown, figure 2 It is the SEM image of the microbial carrier prepared in Example 2 of the present invention.

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Abstract

The invention provides an immobilized microbial system, which comprises a microbial carrier and microorganisms attached to the surface of the microbial carrier, wherein the microbial carrier is prepared by carbonizing plant tissues. The invention also provides a preparation method of the immobilized microbial system and a water toxicity detection method. The preparation method disclosed by the invention, which prepares the immobilized microbial system from the plant carbonized tissues by virtue of a one-step method, is simple to implement; moreover, according to the immobilized microbial system provided by the invention, the microorganisms are immobilized by virtue of an attachment process, so that the microorganisms naturally deposit on pore surfaces of a carrier material, and a cross-linking curing process is avoided; and in addition, the adopted microbial carrier, which is of a three-dimensional spatial reticular structure, is relatively good in mass transfer performance. Therefore, the immobilized microbial system provided by the invention is relatively good in activity in a process of detecting the water toxicity; the immobilized microbial system can be repeatedly applicable to water toxicity detection; and the immobilized microbial system is relatively good in detection effect.

Description

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Claims

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Application Information

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Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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