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Stem cell preparation for treating hepatic injury

A technology of stem cell preparation and liver injury, which is applied in the field of stem cell preparation for the treatment of liver injury. It can solve the problems of difficult cultivation and preservation of liver cells, small number of liver cell proliferation, and difficulty in achieving clinical treatment, so as to promote self-renewal ability and improve liver function. Function, guaranteed activity effect

Inactive Publication Date: 2016-12-07
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hepatocytes are a kind of static cells, and the number of transplanted hepatocytes proliferates is small, so it is difficult to achieve the effect of clinical treatment, and it is difficult to culture and preserve hepatocytes.

Method used

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  • Stem cell preparation for treating hepatic injury
  • Stem cell preparation for treating hepatic injury
  • Stem cell preparation for treating hepatic injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation and detection of embryonic liver cells

[0022] 1. Organization source

[0023] Embryonic liver tissues from 12 to 24 weeks of pregnancy (approved by the Ethics Committee) were selected from clinically normal pregnant women who had spontaneously aborted.

[0024] 2. Primary culture and subculture of embryonic liver cells

[0025] Isolation and primary culture: After the embryos are elicited, they are stored at 4°C and transported to the laboratory. The liver is taken out, the outer membrane is removed, and washed with D-Hank's solution (containing double double antibodies) until the tissue is grayish white. Cut into pieces and move to Centrifuge in a centrifuge tube at 3000r / min for 7min, discard the supernatant, add 15mL of 0.25% trypsin / 0.03% EDTA digestion solution to digest at room temperature for 8min, discard the digestion solution, add 15mL of fresh digestion solution for digestion for 8min, and remove the supernatant Transfer the solution into a...

Embodiment 2

[0034] 1. Preparation of embryonic liver cells

[0035] Please refer to Example 1, which is the same as Example 1.

[0036] 2. Preparation of stem cell preparations

[0037] Take the 3rd passage human embryonic liver cells prepared in step 1 2×10 6 One, mixes with estrogen 400ng, polysaccharide 0.2ml, appropriate amount of water, and makes the stem cell preparation of 20ml. Furthermore, the concentration of human embryonic liver cells was 1×10 5 each / ml, the concentration of estrogen is 20ng / ml, and the volume percentage of polysaccharide is 1%.

Embodiment 3

[0039] 1. Preparation of embryonic liver cells

[0040] Please refer to Example 1, which is the same as Example 1.

[0041] 2. Preparation of stem cell preparations

[0042] Take 1 × 10 human embryonic liver cells of the third generation prepared in step 17 1, mixed with 1000ng of estrogen, 2ml of polysaccharide, and appropriate amount of water to prepare 20ml of stem cell preparation. Furthermore, the concentration of human embryonic liver cells was 5×10 5 each / ml, the concentration of estrogen is 50ng / ml, and the volume percentage of polysaccharide is 10%.

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Abstract

The invention discloses a stem cell preparation for treating liver damage, wherein the stem cell preparation for treating liver damage comprises: embryonic liver cells, estrogen, polysaccharide and solvent, wherein the concentration of the embryonic liver cells is (1-5 )×10 5 each / ml; the concentration of the estrogen is 20-50ng / ml; the volume percentage of the polysaccharide is 1-10%. The stem cell preparation for treating liver injury of the present invention is prepared by blending embryonic hepatocytes, estrogen, polysaccharides and solvents, wherein the embryonic hepatocytes, as a kind of liver stem cells, can effectively repair damaged liver tissue, and at the same time differentiate and proliferate new Estrogen-treated embryonic liver cells secrete more vascular endothelial growth factor and increase the number of endothelial progenitor cells in the blood circulation , which is beneficial to improve the hepatic blood circulation during liver fibrosis; polysaccharides provide nutrients for cells and ensure the activity of cells.

Description

technical field [0001] The invention relates to the technical field of stem cells and tissue engineering, in particular to a stem cell preparation for treating liver damage. Background technique [0002] Liver fibrosis and cirrhosis are difficult liver diseases. Although liver transplantation is an effective treatment for advanced liver cirrhosis, the prognosis of these patients is still poor; and there are problems such as lack of donors, surgical injury, immune rejection and high cost of liver transplantation. Hepatic cell transplantation is considered as one of the alternative methods of organ transplantation, not only can temporarily maintain the liver function of patients waiting for liver transplantation, but also can treat certain liver metabolic diseases. However, hepatocytes are a kind of quiescent cells, and the number of transplanted hepatocytes proliferates is small, so it is difficult to achieve the effect of clinical treatment, and it is difficult to culture a...

Claims

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Application Information

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IPC IPC(8): A61K35/545A61P1/16A61K31/715
CPCA61K35/545A61K31/715
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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