Kit and method for determining triglyceride

A triglyceride and kit technology, applied in the field of medical testing, can solve the problems of delaying the disease, complications, affecting the prognosis of patients, etc.

Active Publication Date: 2017-02-15
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Calcium Dobesilate and Etamine Interfering with Enzymatic Triglyceride Measurements Causes Major Inconvenience for Patients Administered These Two Drugs
For example, for diabetic patients, they are often accompanied by coronary heart disease, vascular arteriosclerosis, etc., and the interference of the administered calcium dobesilate on the enzymatic detection of triglycerides may cause the triglyceride level of diabetic patients to be incorrectly evaluated. cov

Method used

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  • Kit and method for determining triglyceride
  • Kit and method for determining triglyceride
  • Kit and method for determining triglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: Triglyceride Determination Kit Reagent Preparation

[0095] According to the components and concentrations shown in Table 6 and Table 7 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0096] Table 6: R1 formulation

[0097]

[0098] Table 7: R2 formulation

[0099]

Embodiment 2

[0100] Embodiment 2: Triglyceride determination kit reagent preparation

[0101] According to the components and concentrations shown in Table 8 and Table 9 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0102] Table 8: R1 formulation

[0103]

[0104] Table 9: R2 formulation

[0105]

[0106]

Embodiment 3

[0107] Embodiment 3: Triglyceride determination kit reagent preparation

[0108] According to the components and concentrations shown in Table 10 below and Table 11 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0109] Table 10: R1 formulation

[0110]

[0111] Table 11: R2 formulation

[0112]

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PUM

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Abstract

The invention relates to a kit and a method for determining triglyceride. The kit comprises a reagent group 1 and a reagent group 2, wherein the reagent group 1 comprises magnesium salts, triphosadenine, glycerokinase, glycerol lipase oxidase, peroxidase and chromogen; the reagent group 2 comprises lipoprotein lipases and 4-amino-antipyrine, wherein the chromogen is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline (DAOS) or comprises DAOS. The kit and the method provided by the invention have the advantage that the interference caused by calcium dobesilate and/or etamsylate existing in the sample during the triglyceride detection can be reduced.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, the present invention relates to a kit and method for detecting triglycerides. Background technique [0002] Triglyceride (Triglyceride, TG) is a fat molecule formed by long-chain fatty acids and glycerol. It is the most abundant lipid in the human body. Most tissues can use TG decomposition products to supply energy. At the same time, tissues such as liver and fat can also carry out TG synthesis and storage in adipose tissue. [0003] Triglycerides are also the main component of blood lipids and are important energy-supplying substances for the body. Triglycerides in food are digested and decomposed by pancreatic lipase in the intestinal tract, and after absorption, they are re-esterified into triglycerides and enter the blood through the lymphatic system. Under pathological conditions, triglycerides synthesized and stored in various tissue cells increase significantly....

Claims

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Application Information

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IPC IPC(8): C12Q1/61C12Q1/28C12Q1/26C12Q1/44C12Q1/48
CPCC12Q1/26C12Q1/28C12Q1/44C12Q1/485C12Q1/61C12Q2326/96G01N2333/902G01N2333/90235G01N2333/91215G01N2333/918
Inventor 吴昌英
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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