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Application of SNP molecular marker closely linked to aphid resistance gene of prunus persica

A molecular marker and anti-aphid gene technology, applied in the field of molecular biology, can solve the problems of high cost, long research and development cycle of new anti-aphid drugs, and laborious, and achieve the effect of ensuring accuracy

Active Publication Date: 2017-02-22
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development cycle of new anti-aphid drugs is long and the cost is high, and it has become difficult in the race against the evolution of aphids

Method used

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  • Application of SNP molecular marker closely linked to aphid resistance gene of prunus persica
  • Application of SNP molecular marker closely linked to aphid resistance gene of prunus persica
  • Application of SNP molecular marker closely linked to aphid resistance gene of prunus persica

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Embodiment 1

[0033] (1) Identification of aphid-resistant phenotypes and population construction

[0034] (1) Identification of aphid-resistant phenotypes

[0035] 01-77-3 (anti-aphid) was used as the female parent, and Zhongyoutao No. 13 (aphid-sensitive) was used as the male parent for hybridization, manual detasseling and artificial pollination, and 108 seedlings of artificial hybridization progeny were placed in the net room Carry out inoculation treatment, artificially inoculate 10 green aphids, put on a small gauze net, observe the damage of aphids to new shoots, in order to determine the phenotypes of aphid resistance and aphid sensitivity, among them, those with red dots and no other harmful symptoms are aphid resistant types ( figure 1 ), and those with obvious harmful symptoms are the type of aphids ( figure 2 ).

[0036] (2) Identification of segregation phenotypes in hybrid populations

[0037] In the present invention, 01-77-3 (anti-aphid) and Zhongyoutao No. 13 (aphid-su...

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Abstract

The invention discloses application of an SNP molecular marker closely linked to an aphid resistance gene of prunus persica, wherein the molecular marker comprises KyHRM-17-45.71 and KyHRM-3-46.12, which are respectively located at 45.713 Mb and 46.121 Mb of prunus persica genome (Version 2.0) Scaffold 1; alleles KyHRM-17-45.71 are T and G, and the sequence is shown in SEQ ID NO.1; the alleles of KyHRM-3-46.12 are C and G, and the sequence is shown in SEQ ID NO.2. In the invention, the third generation of markup technology based on SNP is used in combination with a constructed isolated hybrid population, and a map-based cloning method is adopted to finely locate the aphid resistance gene of prunus persica; stable, co-dominant and pleomorphic SNP markers are developed in a fine location region, to obtain the marker closely linked to a target trait.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to the application of a SNP marker closely linked with a peach aphid resistance gene. Background technique [0002] Peach [Prunus persica (L.) Batsch] is juicy, delicious and nutritious. It is a traditional fruit loved by people in our country. According to the data of the Food and Agriculture Organization of the United Nations, in 2013, my country's peach cultivation area was 11.66 million mu, and the output was 11.95 million tons, accounting for 50.5% and 55.0% of the world's total cultivation area and total output (FDA). At the same time, peach is also the third largest deciduous fruit tree in my country, which is widely cultivated all over the country and is an important part of agricultural production. [0003] Aphids are Hemiptera insects, including nearly 5,000 species. They are the most diverse, widely distributed, and most harmful herbivorous piercing-sucking i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 牛良鲁振华王志强潘磊张南南崔国朝曾文芳
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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