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Growth velocity-related snp markers in Schizothorax chinensis and their applications

A technology of schizothorax and growth speed, which is applied in the field of SNP markers, can solve the problems that need to be excavated, and achieve the effect of speeding up the cultivation process and speeding up the cultivation process

Inactive Publication Date: 2019-07-26
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no SNPs related to the growth of Schizothorax (body weight, body height, total length, and body length) have been reported, and SNP markers that can be effectively used in the breeding of growth-related traits of Schizothorax remain to be discovered

Method used

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Embodiment Construction

[0026] Embodiments of the technical solution of the present invention will be described in detail below. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and therefore are only examples, rather than limiting the protection scope of the present invention.

[0027] 1.1 Sources of Schizothorax population samples

[0028] The Schizothorax to be tested came from the same batch of 13-month-old Schizothorax artificially bred in the cold-water fish farm in Mount Emei. 114 Schizothorax were randomly selected to determine their phenotypic traits (body weight, height, full length, body length), and the fin rays were clipped and stored in absolute ethanol for genomic DNA extraction.

[0029] 1.2 Extraction of Schizothorax genome DNA to be tested

[0030] Cut 25 mg of the preserved fin rays for DNA extraction, and use the Shanghai Sangong Ezup Column Animal Genomic DNA Extraction Kit to extract genomic DNA, and follow the i...

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) marker relevant to the growth rate of schizothorax prenanti and application thereof. The SNP marker comprises an SNP1 marker and an SNP2 marker; nucleotide sequences are as shown by SEQ ID NO. 1 and SEQ ID NO. 2 in sequence. When the genotypes of SNP1 and SNP2 loci are both homozygous CC, the growth rate of the individual of a to-be-detected schizothorax prenanti is quicker. Individuals of which the genotypes at the SNP1 and SNP2 loci are both the CC are selected for hybridization; the genotypes, at the SNP1 and SNP2 loci, of individuals of a later generation are all the homozygous CC; the individuals of the later generation are all quick growing type ones. The SNP marker provided by the invention is closely relevant to the growth rate of the schizothorax prenanti; a parent can be selected according to a breeding requirement at a fry culture early stage; the marker provided by the invention is utilized to carry out assistant breeding; further, the culture progress of the excellent variety of the schizothorax prenanti is quickened.

Description

technical field [0001] The present invention relates to SNP markers related to the growth rate of Schizothorax and its application, in particular to SNP markers related to the growth rate of Schizothorax, primer pairs and kits for detecting the aforementioned SNP markers, and the aforementioned SNP markers, The application of the primer pair and the kit in the selection and breeding of Schizothorax chrysothorax, and the method for detecting the growth speed of Schizothorax chrysostrum. Background technique [0002] Full mouth Schizothorax ( Schizothorax prenanti ) is one of the important cold-water fishes in my country. Because of its tender meat, delicious taste and high nutritional value, it is favored by consumers and has become a valuable economic fish in the upper reaches of the Yangtze River in my country. my country used to have rich wild resources of Schizothorax, but in recent years, due to overfishing, construction of power stations, water pollution and other prob...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 叶华罗辉周朝伟杨月静张争世向梦斌
Owner SOUTHWEST UNIV
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