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Chromatographic fingerprint spectrum method for identifying three varieties of medicinal dendrobium

A technology of fingerprint and Dendrobium, applied in the field of chromatographic fingerprints for identifying three kinds of medicinal Dendrobium

Active Publication Date: 2017-03-15
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no public report on the use of HPSEC chromatography to establish the chromatographic fingerprint of medicinal Dendrobium polysaccharides

Method used

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  • Chromatographic fingerprint spectrum method for identifying three varieties of medicinal dendrobium
  • Chromatographic fingerprint spectrum method for identifying three varieties of medicinal dendrobium
  • Chromatographic fingerprint spectrum method for identifying three varieties of medicinal dendrobium

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A chromatographic fingerprinting method for the identification of three medicinal Dendrobium species, such as figure 1 shown, including the following steps:

[0041] (1): polysaccharide extraction

[0042] According to the five-point sampling method in the Dabie Mountains of Anhui Province, the fresh stems of the three known medicinal dendrobiums (Dendrobium officinale, Dendrobium candidum, and Dendrobium slender stems) that have been grown for more than three years and obtained by sampling in the Dabie Mountains of Anhui in the same year were removed after wintering. Cut into thin slices according to the types and mix them, then dry them in an oven at 60°C to constant weight, and crush them into powder, extract them with absolute ethanol Soxhlet at 85°C until the reflux liquid is colorless, evaporate the solvent, and extract the obtained Dendrobium medicinal residues Add distilled water according to the ratio of solid to liquid 1:5, and condense and reflux in a water ...

Embodiment 2

[0065] A chromatographic fingerprinting method for the identification of three medicinal Dendrobium species, such as figure 1 shown, including the following steps:

[0066] (1): polysaccharide extraction

[0067] According to the five-point sampling method, three kinds of known medicinal Dendrobium (Dendrobium officinale, Dendrobium officinale, and Dendrobium slender stem) obtained by sampling in the Dabie Mountains of Anhui were mixed according to the species, and then dried in an oven at 55°C to constant weight. and crushed into powder, 80°C absolute ethanol Soxhlet extraction until the reflux liquid is colorless, evaporate the solvent, add the obtained Dendrobium medicinal residues into distilled water according to the ratio of solid to liquid 1:6, and condense and reflux in a water bath at 80°C for 5 times , 3h each time; then, combine the above-mentioned extraction filtrates and concentrate under reduced pressure to obtain a crude polysaccharide solution;

[0068] (2): ...

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Abstract

The invention discloses a chromatographic fingerprint spectrum method for identifying three varieties of medicinal dendrobium. The method comprises the following steps of polysaccharide extraction, wherein a crude polysaccharide solution is acquired; polysaccharide purification and concentration, wherein a purified concentrated polysaccharide solution is acquired; chromatographic analysis, wherein a chromatographic fingerprint spectrum is established; unknown dendrobium variety identification. The method has the advantages that three varieties of dendrobium produced in Dabie Mountain in Anhui Province can be effectively distinguished and identified according to molecular weight distribution of the main medicinal component-dendrobium polysaccharides of the dendrobium, and defects existing in a dendrobium officinale identification method set in the 2015 edition of Chinese Pharmacopoeia and a dendrobium huoshanense identification method set in the 2016 edition of Anhui Provincial Standard can be overcome; due to the fact that the dendrobium officinale identification method and the dendrobium huoshanense identification method are rough overlaying information, counterfeiters can counterfeit the medicinal dendrobium through vulnerabilities of the detection methods, the method is closer to the essence of chemical components and is more detailed information, therefore, the counterfeiters hardly can counterfeit the medicinal dendrobium, or the imitation cost is high.

Description

technical field [0001] The invention relates to the technical field of identification and quality control methods for medicinal plant products of the genus Dendrobium orchidaceae, in particular to a chromatographic fingerprint method for identifying three medicinal dendrobiums. Background technique [0002] The Dabie Mountains in Anhui Province mainly produce 3 kinds of medicinal Dendrobium, the species names are: Dendrobiumhuoshanense C.Z.Tang et S.J.Cheng (also known as Huoshan rice dendrobium), Dendrobium candidumWall.exLindl. ) and Dendrobium moniliforme (Linn.) Sw. (also known as Dendrobium moniliforme). All three are Dendrobium orchidaceae, which are perennial herbaceous plants. They mainly contain active ingredients such as polysaccharides, alkaloids, bibenzyls, and phenanthrenes. The three are jointly declared by the Anhui Provincial Government as the geographical indication product "Dendrobium Huoshan" (the species name "Dendrobium Huoshan" refers to only one speci...

Claims

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Application Information

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IPC IPC(8): G01N30/06
CPCG01N30/06
Inventor 邓辉陈乃富陈存武陈乃东戴军
Owner WEST ANHUI UNIV
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