Method for distant hybridization of cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies

A technology of distant hybridization and bream, applied in fish farming, application, climate change adaptation, etc., to achieve the effect of bright colors, high market value, and beautiful body shape

Active Publication Date: 2017-04-05
湖南岳麓山水产育种科技有限公司
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  • Method for distant hybridization of cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies
  • Method for distant hybridization of cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies
  • Method for distant hybridization of cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies

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Embodiment

[0049] A method for distant hybridization between koi carp of the present invention and the group head bream subfamily, comprising the following steps:

[0050] (1) Three to four months before the breeding season, female koi and male bream with obvious sexual maturity, good physical signs, bright color, strong physique and good development were selected as the female parent fish of distant hybridization and The male parent fish is cultivated in a special pond, fed with refined bait 1 to 2 months before breeding, and stimulated by running water every 1 to 3 days to promote the maturation of the gonads of the brood fish;

[0051] (2) From mid-May to early June of the same year, when the water temperature is stable above 20°C, inject luteinizing hormone-releasing hormone analog (LRH-A) and chorion stimulating The mixed oxytocic agent of gonadal hormone (HCG) is used to induce labor, the dosage of LRH-A is 7-10ug / kg, and the dosage of HCG is 600-800IU / kg; after 4 hours, inject the...

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Abstract

The invention discloses a method for distant hybridization of cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies. The method comprises the following steps: taking cyprinuscarpiohaematopterus as female parent fish and megalobramaamblycephala as male parent fish, artificially inducing spawning of the female parent fish and the male parent fish in a breeding season, carrying out dry artificial insemination, incubating obtained fertilized roe in running water in an incubation tank to obtain hybrids of the cyprinuscarpiohaematopterus and the megalobramaamblycephala, and testing and screening the hybrids to obtain diploid cyprinuscarpiohaematopterus, diploid common-carp and crucian-carp hybrids and diploid crucian carp. The method for distant hybridization of the cyprinuscarpiohaematopterus and megalobramaamblycephala subfamilies integrates the excellent characters of the cyprinuscarpiohaematopterus and the megalobramaamblycephala, enriches the fish species on the market, lays a favorable foundation for selection and breeding of excellent species, provides precious resources for follow-up selection and breeding of novel polyploid fish and is of great biological significance in biological evolution and genetic breeding of fish.

Description

technical field [0001] The invention belongs to the field of fish hybridization, in particular to the field of distant hybridization among subfamilies of Cyprinidae fishes, and in particular to a method for distant hybridization between koi carps and squid bream subfamilies. Background technique [0002] Hybridization between species or above is generally called distant hybridization, which is a common method for improving fish traits. As an important method of fish genetic breeding and trait improvement, distant hybridization can integrate a whole set of exogenous genes, thereby changing the expression regulation of hybrid progeny genes, so that hybrid progeny may improve in growth speed, stress resistance, disease resistance and Meat quality and other aspects showed heterosis. For example, the hybrid offspring formed by the intergeneric distant hybridization of red crucian carp and Xiangjiang wild carp has the advantages of beautiful body, fast growth, delicious meat, str...

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 刘少军王余德陶敏覃钦博罗凯坤肖军赵如榕
Owner 湖南岳麓山水产育种科技有限公司
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