Method for distant hybridization between bighead carp and megalobrama amblycephala subfamilies

Abstract

The invention discloses a method for distant hybridization between bighead carp and megalobrama amblycephala subfamilies. The method comprises the following steps of performing artificial spawning induction by taking bighead carp as a female parent and megalobrama amblycephala as a male parent, firstly injecting an oxytocic PG or LRH-A into parent fish of the female parent, and simultaneously injecting the parent fish of the male parent to reduce the dosage by half; injecting a mixed oxytocic consisting of LRH-A, PG and HCG into the parent fish of the female parent after 3-4 hours; and carrying out artificial dry insemination on eggs obtained after artificial spawning induction and sperms, then incubating fertilized eggs with running water, and obtaining filial generations of bighead carpsand megalobrama amblycephala after incubation is successful. The obtained new filial generation provides new resources and basis for genetic breeding of freshwater fish, the whole hybridization process is simple to operate, the breeding period is short, the hatching rate and survival rate of the filial generation are high, and the method has important significance in heritage breeding and industrialization.

Description

technical field [0001] The invention belongs to the field of fish hybridization, in particular to the field of hybridization among subfamilies of fish, in particular to a method for distant hybridization between subfamilies of bighead carp and bighead bream. Background technique [0002] There are more than 32,000 species of fish in the world, which is the most diverse group of vertebrates; among them, the feeding habits of the vast majority of fish are omnivorous or carnivorous; only 3 species of herbivorous fish have been found, which are bream, Grass carp and milkfish. Since the feed of herbivorous fish comes from nature and belongs to the source of vegetable protein, the value of green ecological breeding is getting more and more attention. Distant hybridization refers to the hybridization of interspecies, intergenus and even more distant biological types, which is considered to be an effective way to promote the adaptive radiation evolution and speciation of animals an...

AI Technical Summary

Problems solved by technology

However, bighead carp and bream do not live in the same waters, the reproductive cycle is not synchronized, and the living water environment is different. It is difficult to obtain hybrid offspring smoothly through conventional methods. The rate is very low, it is difficult to realize industrialization promotion

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