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A marker, its preparation method, labeling method and application

A marking method and marker technology, applied in the marker field

Active Publication Date: 2020-11-03
上海医药临床研究中心 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the labeling can only be applied to polysaccharides from glycoproteins that still carry amino groups instead of the usual free sugars

Method used

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  • A marker, its preparation method, labeling method and application
  • A marker, its preparation method, labeling method and application
  • A marker, its preparation method, labeling method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 Synthesis of markers of the present invention

[0060] The marker of the present invention is synthesized as follows:

[0061] Mix equimolar free 4-aminomethyl pyridine (4-aminomethylpyridine, abbreviated as: AMP) and phthalic anhydride, then heat to 140-160°C until no water is produced (about 30 minutes), and cool to the residue Compound A was obtained by recrystallization from methanol.

[0062] Dissolve 0.01 mol of compound A in a mixture of 20 mL of methanol and 5 mL of alkyl iodide, put the mixture into a reflux condenser and continue the reaction until the reaction is complete (4-72 hours). The solution was cooled, the solid was filtered off, and compound B was obtained by recrystallization from methanol.

[0063]

[0064] Compound B was put into 20 mL of 48% HBr, and the mixture was put into a reflux condenser to react until a large amount of phthalic acid precipitated. The solution was cooled to room temperature, 20 mL of water was added, and ...

Embodiment 2

[0067] Embodiment 2 labeling steps

[0068] Sugars can be labeled in two different ways, as shown in the following reactions.

[0069]

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Abstract

The invention provides a marker as well as a preparation method, a marking method and applications thereof. The marker is 1-alkyl-4 amino methyl pyridine. The invention further provides the preparation method and the applications of the marker. The marker is particularly applicable to marking of saccharides, mass spectrum signals can be enhanced, and the mass spectrum detection sensitivity can be improved.

Description

technical field [0001] The present invention relates to a marker, more specifically to a carbohydrate marker, its preparation method and application. Background technique [0002] As we all know, monosaccharides and polysaccharides are the most basic and important molecules that constitute life. In the fields of life sciences such as energy metabolism, nutrition, glycobiology, biopharmaceuticals, and emerging glycomics, with the deepening of experiments, the demand for the discovery and structural characterization of sugars is also becoming more and more apparent. However, since the characteristics and structure of sugar molecules are far more complex than those of proteins and amino acids, the analysis of sugar molecules is still not a simple process even in today's advanced analysis technology. [0003] In general, current analytical procedures rely mostly on chemical labeling. The use of chemical labeling can achieve the following goals: most sugars are hydrophilic and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D213/38C07H5/06C07H1/00G01N30/72
CPCC07D213/38C07H1/00C07H5/06G01N30/7233
Inventor 杨文初王俊霞阮亮亮范锦立甘荣兴
Owner 上海医药临床研究中心
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