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A kind of polyacrylamide gel preparation method that prevents solution from leaking

A technology of polyacrylamide gel and solution, which is applied in the direction of instruments, measuring devices, scientific instruments, etc. It can solve the problems of the bottom of the two glass plates not being tightly sealed, the acrylamide solution leaking, and the assembly of the glass plates not in place, etc., to ensure The effect of agarose gel activity, strong practicability, and less facilities

Active Publication Date: 2019-04-19
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, in the process of making glue, often due to various reasons, such as outdated equipment, improper assembly of glass plates, etc., the bottom of the two glass plates is not tightly sealed, causing the acrylamide solution to leak from the bottom of the glass plate before it solidifies into a gel. Glue failed

Method used

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  • A kind of polyacrylamide gel preparation method that prevents solution from leaking

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Embodiment 1

[0021] The present embodiment is a method for preparing polyacrylamide gel for preventing solution leakage, figure 1 Shown are the glue-making equipment, equipment and experimental conditions constructed and formed in this example.

[0022] Equipment: ① a pipette with a volume of 1 mL, ② a 1 mL tip, ③ a 1% agarose gel solution, and ④ an assembled glass plate for preparing polyacrylamide gel.

[0023] A constant temperature shaking incubator was used as an incubator, and the temperature was adjusted to 50°C, but its shaking function was not used.

[0024] During the operation, first take out ①~④ preheated at 50°C, that is, a pipette with a range of 1mL, a 1mL pipette tip, a 1% agarose gel solution, and a glass plate, and operate at room temperature.

[0025] First, install the pipette tip with one hand and draw 400 μL of 1% agarose solution, hold the front glass 1 and rear glass 2 horizontally with the other hand, and then align the pipette tip to the front glass 1 and Quickl...

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Abstract

The invention relates to a polyacrylamide gel preparation method capable of preventing solution leakage, and belongs to the technical field of physical or chemical apparatus. The bottoms of two pieces of glass are sealed by agarose gel; two pieces of glass are placed on two tables with a same height, two ends, which are connected to each table, of each glass are horizontal, a gap exists between the lower end of each glass and the surface of an operation table, a gel forming area is formed in the gap above the bottoms of two pieces of glass, and gel forming raw materials are added into the gel forming area to start gel production. The preparation method is used to prepare polyacrylamide gel, has the advantages of simpleness, convenience, and rapidness, and is capable of reducing solution waste and guaranteeing the number of gel blocks.

Description

technical field [0001] The invention relates to a preparation method of polyacrylamide gel for preventing solution leakage, belonging to the technical field of physical or chemical devices. Background technique [0002] Polyacrylamide (poly-acrylamide) gel electrophoresis (gel electrophoresis), referred to as PAGE, is the most common and most basic method for qualitative and quantitative analysis of a small amount of protein or nucleic acid in molecular biology research. Sodium dodecyl sulfate (SDS) is added to the gel, that is, SDS-PAGE. If SDS is not added, it is Native PAGE. For general protein detection experiments, currently laboratories generally use the micro vertical PAGE systems of Shanghai Tianneng and Bole Corporation of the United States, and use glass plates and spacers to prevent leakage of acrylamide solution during gel production. However, in the process of making glue, due to various reasons, such as outdated utensils, improper assembly of glass plates, etc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
CPCG01N27/44747
Inventor 蒋桂瑾宋源普李玉兰杨仙玉贾宇坤刘怡君
Owner ZHEJIANG FORESTRY UNIVERSITY
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