Method of inducing and differentiating human umbilical cord derived mesenchymal stem cells into cartilage cells

A technology of mesenchymal stem cells and induced differentiation, applied in the field of induced differentiation of stem cells, can solve the problems of latent virus and pathogenic microorganism infection and low probability of transmission

Inactive Publication Date: 2017-05-10
中卫华医(北京)生物科技有限公司 +1
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  • Claims
  • Application Information

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Problems solved by technology

④Infection and transmission of latent viruses and pathogenic microorganisms are relatively low

Method used

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  • Method of inducing and differentiating human umbilical cord derived mesenchymal stem cells into cartilage cells
  • Method of inducing and differentiating human umbilical cord derived mesenchymal stem cells into cartilage cells
  • Method of inducing and differentiating human umbilical cord derived mesenchymal stem cells into cartilage cells

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Embodiment Construction

[0030] The method of the present invention for inducing differentiation of human umbilical cord-derived mesenchymal stem cells into chondrocytes is to clean the umbilical cord tissue to remove blood stains and cut it into small sections, cut open the small section of umbilical cord to remove vascular tissue, cut up the small section of umbilical cord and digest it to obtain a single mesenchymal stem cell, The obtained mesenchymal stem cells were amplified and cultured to the seventh passage to obtain a single cell suspension, inoculated in serum-free medium containing chondrogenic differentiation inducer to continue culturing, half of the fresh medium was replaced every 3 days, and after 14 days of induction , to obtain chondrocytes.

[0031] Specifically, the preparation of the single-cell suspension is carried out first (1) pretreatment of the umbilical cord tissue, followed by (2) enzyme digestion method to obtain primary MSCs cells from the umbilical cord, and then (3) umbi...

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Abstract

The invention relates to a method of inducing and differentiating human umbilical cord derived mesenchymal stem cells into cartilage cells. The method includes: in order to solve the problem that the prior art is low in differentiation efficiency, cleaning umbilical cord tissue to remove blood stain, cutting the same into small sections, dissecting the small sections to remove vascular tissue, cutting the small sections into pieces, and digesting to obtain a single mesenchymal stem cell; culturing the obtained mesenchymal stem cell in an amplification manner to the seventh generation to obtain a unicellular suspension, inoculating the unicellular suspension into a serum-free culture medium containing a cartilage differentiation inducer to continue culturing, replacing a fresh culture medium every other 3.5 days, and inducing for 14 days to obtain the cartilage cells. The method can efficiently differentiate the umbilical cord mesenchymal stem cells into the cartilage cells and is of important significance in the aspects of restoring cartilage tissue in human bodies and treating bone injury.

Description

technical field [0001] The invention relates to a method for inducing differentiation of stem cells, in particular to a method for inducing the differentiation of human umbilical cord-derived mesenchymal stem cells into chondrocytes. Background technique [0002] Knee cartilage injury is one of the main research fields of sports medicine clinical work, and it is a hot spot and focus of sports science research. In the early stage of articular cartilage damage, it can cause pain and limitation of joint function of the injured joint. In the late stage, the joint may have degeneration and non-inflammatory osteoarthritis, which will seriously reduce the quality of life of the patient. After articular cartilage is damaged, chondrocytes mainly rely on synovial fluid and the matrix around the cells to provide nutrition, and supply energy through anaerobic glycolysis. Their own repair ability is very limited, and they cannot even be repaired by themselves. [0003] The emergence of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0655C12N2500/24C12N2500/30C12N2500/38C12N2501/15C12N2506/1392
Inventor 李亚平吴志宏熊开宇何辉王晶谷广其邱丽媛房芳赵进军何文丽
Owner 中卫华医(北京)生物科技有限公司
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