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Heavy metal detection method based on aptamers-light addressable potentiometric sensors

A potential sensor and detection method technology, applied in the field of electrochemical detection, can solve the problems of mercury electrode toxicity, environmental secondary pollution, etc., and achieve the effect of high sensitivity detection

Inactive Publication Date: 2017-05-10
NANKAI UNIV +2
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Problems solved by technology

[0012] The present invention provides a kind of "heavy metal detection method based on aptamer-light addressable potential sensor", the method uses light addressable potential sensor in array layout as substrate, different types of heavy metal ion aptamer probes Fixed on the upper surface of each optical addressable sensor, it can realize heavy metal ion detection with high sensitivity, high reliability and high resolution of various heavy metal ions, and can solve the toxicity of traditional electrochemical mercury electrodes and secondary pollution to the environment, etc. question

Method used

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Embodiment Construction

[0022] Step 1: Functional treatment of the light-addressable sensor substrate. First soak the light-addressable sensor with piranha solution, then rinse with ultrapure water, and then use APTES for silanization treatment. Finally, heavy metal aptamers with amino groups at the ends are immobilized The probes are fixed on the upper surface of the photo-addressable sensor substrate.

[0023] Step 2: Pretreatment of the original water body sample, that is, after the original sample is adsorbed and filtered by activated carbon, the heavy metal ions in it are enriched by the functionalized ultrafine fiber membrane, and then the metal ions are eluted to form a solution to be tested.

[0024] Step 3: Quantitative detection, load the aptamer-light addressable potential sensor on the "encapsulation probe of photoelectric biochemical semiconductor sensor [34]", inject the solution to be tested into the upper surface of the chip, and measure the output current of the light addressable pote...

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Abstract

The invention provides a heavy metal detection method based on aptamers-light addressable potentiometric sensors. According to the method, the light addressable potentiometric sensors arranged in arrays are taken as a substrate, different kinds of heavy metal ion aptamer probes are fixed on upper surfaces of the light addressable potentiometric sensors, so that high-sensitivity, high-reliability and high-resolution heavy metal ion detection of multiple kinds of heavy metal ions can be realized, and the problems that a traditional electrochemical mercury electrode is toxic, causes secondary pollution to the environment and the like can be solved.

Description

[0001] [Technical Field] The present invention belongs to the field of electrochemical detection. [0002] 【Background technique】 [0003] Heavy metals refer to metals with a specific gravity greater than 5, which can cause chronic poisoning if they accumulate to a certain extent in the human body. Heavy metals mainly include 45 kinds of Au, Ag, Cu, Pb, Ni, Co, Cr, Hg, Cd, As, etc. The most harmful to the human body are Pb, Hg, Cd, As, Cr, etc. [1,2]. Therefore, the research on heavy metal detection technology usually takes these five heavy metals as the target model. There are many traditional heavy metal detection technologies, which have the advantages of high detection accuracy and wide range. At present, the laboratory evaluation system of various countries has been formed; however, the traditional heavy metal detection equipment is expensive and not easy to carry. Therefore, it still cannot meet the requirements of modern water environment detection. Among them, there is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 贾芸芳范清杰
Owner NANKAI UNIV
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