Method of Transplanting Cotton Test-tube Seedlings in Ditch
A technology of test-tube seedlings and trenches, which is applied in the fields of cotton cultivation, horticultural methods, botany equipment and methods, etc., and can solve the problems of low survival rate, etc.
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Embodiment 1
[0033] Embodiment 1, cotton test-tube seedling trench transplanting method
[0034] 1. Preparation of trenches and grafts
[0035] 1) Trench digging
[0036] In the summer of July in Yuncheng, Shanxi, a ditch with a length of about 200 cm, a width of 80 cm, and a depth of 100 cm was dug under the shade shed of the Cotton Research Institute of the Shanxi Academy of Agricultural Sciences (under an ordinary rain-proof shade shed). The temperature in the ditch was 25 ℃, the humidity in the trench is about 80%, and the light intensity in the trench is about 1500LX.
[0037] 2), graft
[0038] Select sea-island cotton seedlings with 3-4 true leaves in flower pots as rootstocks, use 50 Zhongmian No. 35 tissue culture regeneration cotton test tube seedlings (use the hypocotyls of Zhongmian 35 sterile seedlings as explants) Tissue culture, the cotton regenerated plants obtained through the embryonic differentiation pathway) are respectively used as scions for grafting, and the cotto...
Embodiment 2
[0048] Embodiment 2, cotton test-tube seedling trench transplanting method
[0049] The method is basically the same as in Example 1, except that 50 Zhongmian No. 35 tissue-cultured regenerated cotton test-tube plantlets are replaced by 30 Simian No. 3 tissue-cultured regenerated cotton test-tube plantlets.
[0050] As a result, 30 test-tube plantlets were grafted in the test, and 18 survived, with a survival rate of 60%.
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