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Novel application of artesunate

A new technology of artesunate, applied in the field of lung cancer treatment, can solve the problems of drug resistance and limited application of molecular targeted drugs, and achieve the goal of reversing erlotinib resistance, solving drug resistance and improving the prognosis of lung cancer Effect

Pending Publication Date: 2017-05-31
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a new application of artesunate to solve the problem that existing molecular targeted drugs will eventually produce drug resistance and limit its clinical application

Method used

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  • Novel application of artesunate
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  • Novel application of artesunate

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0026] Antitumor activity of artesunate on human lung adenocarcinoma PC9 cells in vitro

[0027] 1 Materials and methods

[0028] 1.1 Main experimental materials

[0029] 1.1.1 Cell lines

[0030] Human lung adenocarcinoma PC9 cells were kindly donated by Professor Chen Zhengtang, Army Tumor Institute, Xinqiao Hospital, Third Military Medical University.

[0031] 1.1.2 Main reagents

[0032]

[0033] 1.1.3 Main materials and equipment

[0034]

[0035]

[0036] 1.2 Experimental method

[0037] 1.2.1 Recovery, culture, subculture and cryopreservation of cells

[0038] Tumor cell recovery and culture

[0039] Take out the cryopreservation tube containing the human lung adenocarcinoma PC9 cell line from the liquid nitrogen tank, put it into a beaker filled with warm water at 37°C-40°C and shake it quickly, so that the frozen cell solution can be quickly dissolved within 30s, and the cells Transfer to a sterile centrifuge tube, add 5ml of RPMI-1640 culture medium (...

experiment example 2

[0107] In Vitro Experiment of Artesunate Reversing Erlotinib Resistance in PC9 / ER Cells

[0108] 1 Materials and methods

[0109] 1.1 Main experimental materials

[0110] 1.1.1 Main reagents

[0111] LY294002 was first formulated into a 20nmol / L mother solution (dissolved in DMSO), stored in a -20°C refrigerator for a long time, and the working solution was prepared with fresh cell culture medium before use as required by the experiment.

[0112] 1.2 Experimental method

[0113] 1.2.1 The experimental methods of cell recovery, culture, subculture and cryopreservation are the same as before.

[0114] 1.2.2 The cck8 kit was used to detect the effect of ART on the proliferation ability of PC9 / ER cells.

[0115] PC9 cells and PC9 / ER cells in the logarithmic growth phase were mixed at 5×10 per well 3 Cell density, seeded in 96-well plate, 100 μl per well, placed at 37°C, 5% CO 2 After culturing in the incubator for 24 hours, ART was added to make the final concentrations of 6...

experiment example 3

[0138] In Vivo Experiment of Artesunate Reversal of Erlotinib Resistance in PC9 / ER Cells

[0139] 1 Experimental method

[0140] 1.1 Establishment of experimental animal tumor models

[0141] Select a flask of PC9 / ER cells in the logarithmic growth phase, then digest the cells with 0.25% trypsin 1-2ml containing 0.025% EDTA, observe under an inverted microscope, and quickly add a small amount of 10% FBS when the cells withdraw their protrusions and become round Gently blow and blow the digested cells repeatedly to separate them from the culture flask wall and disperse them into a cell suspension. Then transfer the cells into a new centrifuge tube and centrifuge on a centrifuge (1000rpm, 5min). The white cell pellet was washed 2-3 times with PBS solution, counted with a cell counting plate, and then the cell concentration was adjusted to 1×10 with fresh culture medium (without FBS). 7 / ml, sent immediately to the SPF grade animal breeding room under sterile conditions. Injec...

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Abstract

The invention provides novel application of artesunate. The artesunate is used for reversing erlotinib drug resistance of lung adenocarcinoma, or the artesunate is used together with erlotinib. The problem that clinical application of a conventional molecular targeted drug is limited as drug resistance can be resulted ultimately can be solved. The invention belongs to the field of lung cancer treatment.

Description

technical field [0001] The invention relates to a method for reversing drug resistance of lung adenocarcinoma to erlotinib, belonging to the field of lung cancer treatment. Background technique [0002] Lung cancer is the main cause of cancer-related death. The emergence of molecular targeted therapy has brought new hope for the treatment of lung cancer. It has a clear site of action, and has few adverse reactions and definite curative effect. The disadvantages of molecular targeted drugs are that they are expensive, cannot completely clear the lesion, and eventually develop drug resistance, which limits their clinical application. . Contents of the invention [0003] The purpose of the present invention is to provide a new application of artesunate to solve the problem that existing molecular targeted drugs will eventually produce drug resistance and limit its clinical application. [0004] In order to solve the above problems, a new application of artesunate is propose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/517A61K31/357A61P35/00
CPCA61K31/357A61K31/517A61K2300/00
Inventor 马虎周建国张钰柏玉举吕水萍刘大海岳国军石磊张廷友沈刚
Owner AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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