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Application of seaweed oligosaccharide in preparation of avian leucosis virus resistant preparation

A technology of avian leukosis virus and seaweed oligosaccharides, which is applied in the field of biomedicine, can solve the problems that avian leukosis virus has not been reported, and achieve the effects of anti-avian leukemia virus immunity, improving immunity, and rich resources

Active Publication Date: 2017-05-31
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its use in resisting avian leukosis virus has not been reported yet

Method used

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  • Application of seaweed oligosaccharide in preparation of avian leucosis virus resistant preparation
  • Application of seaweed oligosaccharide in preparation of avian leucosis virus resistant preparation
  • Application of seaweed oligosaccharide in preparation of avian leucosis virus resistant preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Taking Centipede, Ulva vulgaris and Hijiki as examples to extract seaweed polysaccharides:

[0023] Centipede algae ( Grateloupia filicina ) Wash, dry at 50°C to a constant weight, break, take 10g of the broken centipede algae, add 600mL distilled water, and extract for 4 hours in a water bath at 100°C; filter the residue with 100 mesh, 200 mesh and 300 mesh sieves respectively After filtration, the filtrate was dialyzed to remove salt and concentrated under reduced pressure to 1 / 10 of the original volume, added with 3 times the volume of the concentrated solution and alcohol-precipitated for 24 hours, centrifuged, precipitated and lyophilized to obtain centipede algae polysaccharide, ready for use.

[0024] Will Kong Ulva ( Ulva Pertusa ) Wash, dry at 50℃ to a constant weight, crush, take 10g of the crushed Ulva perforatum, add 400mL distilled water, and extract in a water bath at 125℃ for 4 hours; use 100 mesh, 200 mesh and 300 mesh sieves respectively The filter residue...

Embodiment 2

[0030] In vitro anti-avian leukemia virus experiment

[0031] Reagent preparation instructions:

[0032] Cell growth medium: 10mL fetal bovine serum is added to 90mL DMEM medium.

[0033] Cell maintenance solution: 2mL fetal bovine serum was added to 98mL DMEM medium.

[0034] PBS buffer: take 8gNaCl, 0.2gKCl, 3.58g Na 2 HPO 4 ·12H 2 O, 0.27g KH 2 PO 4 Dissolve in 1L double-distilled water, adjust the pH to 7.2-7.4, pass through three layers of 0.22μm membranes, and sterilize.

[0035] 1) Cytotoxicity test

[0036] Chicken embryo fibroblasts (DF-1) were plated on a 96-well plate. After growing to a monolayer in the cell growth medium, the culture medium was discarded, washed three times with PBS, and the cell maintenance medium was added to each well. 100μL of each seaweed oligosaccharide solution with concentrations of 2, 1, 0.5, 0.25, 0.125, 0.0625, 0.03125mg / mL, three replicates for each concentration, and a cell control group (100μL cell maintenance solution added to monolayer cells...

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Abstract

The invention belongs to the technical field of biomedicine and particularly relates to application of seaweed oligosaccharide in the preparation of an avian leucosis virus resistant preparation. seaweed oligosaccharide is prepared by degrading natural polysaccharides extracted from the macroalga Grateloupia filicina, Ulva lactuca, Sargassum fusiforme and other algae, has a wide source range, is simple to prepare, and has the advantages, as an antiviral preparation, such as naturalness, zero toxicity and environmental friendliness; in-vitro cell experiments prove that the seaweed oligosaccharide is capable of inhibiting the proliferation of avian leucosis virus, has significant antiviral effect, may be applied to livestock and poultry breeding as a novel antiviral preparation, and has high applicable value.

Description

Technical field [0001] The invention relates to the field of biomedicine technology, in particular to the application of seaweed oligosaccharides in the preparation of anti-avian leukemia virus preparations. Background technique [0002] Avian leucosis (Avian leucosis) is a general term for a variety of neoplastic diseases caused by the retroviridae (Retroviridae) avian leukosis / Sarcoma virus group (Avian leukosis / Sarcoma virus, ALV), which is dominated by the malignant proliferation of hematopoietic cells. According to clinical symptoms, it can be divided into lymphocytic leukemia, erythroblastic leukemia, myeloblastic leukemia, myeloid leukemia, connective tissue tumor, bone sclerosis and so on. Since ALV was first reported and isolated in 1908, it has spread and occurred in many countries around the world, causing huge economic losses to the breeding industry. At present, there are still no suitable drugs and vaccines to prevent and treat the occurrence and prevalence of ALV,...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61K31/737A61P35/02A61P35/00A61P31/14
CPCA61K31/702A61K31/737A61K2300/00
Inventor 李鹏程孙雨豪陈晓琳刘松于华华邢荣娥李荣锋王雪芹
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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