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Method for quantitative determination of serratia marcescens

A technology of Serratia marcescens and a quantitative detection method, which is applied in the field of detection of indicator bacteria for evaluating the protective performance of personnel biological protective equipment

Active Publication Date: 2017-05-31
NAT INST OF METROLOGY CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there is still little research on the quantitative detection method of Serratia marcescens, so it is very necessary to establish an accurate and reliable quantitative detection method and uncertainty evaluation method of Serratia marcescens

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  • Method for quantitative determination of serratia marcescens
  • Method for quantitative determination of serratia marcescens
  • Method for quantitative determination of serratia marcescens

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Embodiment Construction

[0079] Quantitative detection of Serratia marcescens in freeze-dried samples

[0080] 1. Materials and methods

[0081] 1. The number of the standard strain of Serratia marcescens is ATCC8039; the sample to be tested is Serratia marcescens and the concentration range of the lyophilized sample is (0.87~1.27)×10 10 CFU / ml (developed by China Institute of Metrology); according to the composition of nutrient agar medium (beef extract 3.0g, peptone 5.0g, yeast powder 3.0g, sucrose 10.0g, agar powder 15.0g, distilled water 1000mL) to prepare the medium, Boil to dissolve, adjust the pH to 7.2±0.2, and autoclave at 121°C for 15 minutes. Heat to melt the agar before use, shake well and pour the plate.

[0082] 2. Take the freeze-dried sample to be tested out of the -20°C refrigerator, place it at room temperature for 5 minutes, add 2 mL of sterile phosphate buffer with a pipette as a diluent for rehydration and dissolution, mix well and prepare S 0 Sample homogenate. Then use a 1m...

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Abstract

The invention discloses a method for quantitative determination of serratia marcescens. A serratia marcescens culture detection method is provided, a sampling loading volume best for uniform distribution of serratia marcescens on a nutrient agar plate is found by optimizing the sample loading volume in a plate count method, and the accuracy of a final plate count result is guaranteed. A computational formula suitable for a serratia marcescens quantitative determination result is also provided. Through analysis of the quantitative determination method and parameters in the detection result computational formula, the invention further provides an uncertainty evaluation method for quantitative determination of serratia marcescens. Serratia marcescens is an evaluation indicator bacterium for protective property of biological protection equipment for people, and evaluation accuracy and comparability can be guaranteed only through accurate quantitative determination.

Description

[0001] Technical field: [0002] The invention relates to the detection of indicator bacteria for protection performance evaluation of personnel biological protective equipment, in particular to establishing an accurate quantitative detection method for Serratia marcescens. The invention also relates to establishing an uncertainty evaluation method for the quantitative detection of Serratia marcescens. [0003] Background technique: [0004] Biological safety cabinets, biological protective clothing, biological protective masks, and medical surgical masks are common biological protective equipment. In order to ensure the safety of operators, it is necessary to evaluate the microbial protection performance of these equipment. Serratia marcescens (Serratia marcescens) is recognized as an indicator bacterium for biocontainment evaluation because of its short culture period, good resistance to atomization and impact, and easy disinfection treatment. Therefore, the accuracy of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12R1/43
CPCC12Q1/06C12Q1/10
Inventor 隋志伟王晶刘晓夏薛蕾
Owner NAT INST OF METROLOGY CHINA
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