SSR markers, closely linked to rice black-streaked dwarf disease resistance QTL, on chromosome 4 and application of SSR marker

Abstract

The invention relates to SSR markers, closely linked to rice black-streaked dwarf disease resistance QTL, on a chromosome 4 and application of the SSR markers. According to the SSR markers, closely linked to rice black-streaked dwarf disease resistance QTL, on the chromosome 4, a filial generation F2 population of rice black-streaked dwarf disease resistant variety Zhejing 5 and susceptible variety Lianyuangang 3 is used as a mapping population; the rice black-streaked dwarf disease resistance is identified by using a combined method of natural field identification and improved laodelphax striatellus antixenosis identification; linkage analysis is carried out on disease resistance identification results and SSR marker data to obtain the two SSR markers, closely linked to rice black-streaked dwarf disease resistance major QTL, on the chromosome 4; and the resistance of a plant to the rice black-streaked dwarf disease can be predicated by detecting banding pattern data of the primer markers on the chromosome 4. Therefore, the selecting efficiency of black-streaked dwarf disease resistant rices is greatly enhanced and the breeding progress is accelerated.

Description

technical field [0001] The invention relates to an SSR marker closely linked with a rice black-streaked dwarf resistance QTL on chromosome 4 and an application thereof, belonging to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf disease is a viral disease caused by Rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by the small planthopper. There are 28 species of hosts including hybrid rice, conventional rice, barley, wheat, corn, millet, sorghum, kangaroo, bluegrass, barngrass, barnyardgrass, and matang. Rice black-streaked dwarf virus infects corn and causes corn rough dwarf disease, and infects wheat to cause wheat dwarf disease. The pathogenic forms, hosts and symptoms, vector insects and disease transmission characteristics of these diseases are very similar. Rice black-streaked dwarf disease can occur at the rice seedling stage, tillering stage, and jointing stage. Due to different infection...

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to provide a QTL closely linked with rice black-streaked dwarf resistance on chromosome 4 for the shortcomings of rice black-streaked dwarf resistance identification stability, poor repeatability, time-consuming and labor-intensive in conventional breeding. SSR markers and their application, the method can predict the resistance of plants to rice black-streaked dwarf disease, simplify the selection method, and accelerate the breeding process of disease-resistant varieties

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