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Preparation method and application of highly active anti-tumor peptide of Philippine clam

A Philippine clam, high-activity technology, applied in the direction of peptide preparation methods, antineoplastic drugs, chemical instruments and methods, etc., can solve the problems of low purity and activity, and achieve easy popularization and application, strong tumor suppression effect, and less investment Effect

Active Publication Date: 2020-10-09
FISHERIES RES INST OF FUJIAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In October 2016, our institute published the "Research on the Preparation of Anti-tumor Peptides of Philippine Clams by Enzyme Method" ("Fishery Modernization", Volume 43, Issue 5). The peptide product has a high inhibitory rate on A549 in vitro, but its purity and activity are not high, and only active peptides can produce good effects on the human body. Therefore, it is necessary to conduct further research and development to improve the anti-tumor effect of Philippine clams. peptide activity

Method used

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  • Preparation method and application of highly active anti-tumor peptide of Philippine clam
  • Preparation method and application of highly active anti-tumor peptide of Philippine clam
  • Preparation method and application of highly active anti-tumor peptide of Philippine clam

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Embodiment Construction

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0025] This example discloses the preparation and analysis process of the highly active anti-tumor peptide of Philippine clam, and the process of carrying out the mouse animal experiment of the prepared product, as follows:

[0026] 1. Material preparation

[0027] (1) Animals: Philippine clams were purchased from a supermarket, sanded for 1 day, washed, shelled, fleshed, homogenized, and stored at -20°C for later use; SPF grade Balb / c-nu grade nude mice, half male and half male , 12-15g, 4-5 weeks old, 40 rats, provided by Guangdong Medical Experimental Animal Center.

[0028] (2) Cell line: A549 non-small lung cancer cells were purchased from the Basic Medical Cell Center of the Institute of Basic Medical Sciences, Chin...

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Abstract

The invention discloses a preparation method of ruditapes philippinarum high-activity anti-tumor peptide. The preparation method comprises such steps as conducting enzymatic hydrolysis, conducting ultra-filtration, conducting strong cation exchange column chromatographic separation, conducting gel column chromatographic separation and the like; and in addition, the invention discloses an application of the ruditapes philippinarum high-activity anti-tumor peptide, which is prepared by virtue of the preparation method, in inhibiting A549 non-small cell lung cancer. According to the preparation method, the ruditapes philippinarum anti-tumor peptide is prepared by virtue of optical enzymatic hydrolysis and purification processes, a component of which molecular weight is less than 5 KDa is obtained by conducting ultra-filtration, and are then subjected to separation and purification in two steps, so that the high-activity polypeptide is obtained; and the preparation method is simple in overall process and easy for popularization and application.

Description

technical field [0001] The invention relates to the technical field of aquatic product processing and utilization, in particular to a preparation method and application of an anti-tumor peptide from Philippine clams. Background technique [0002] Bioactive peptides are inactive specific protein fragments within the protein sequence. This specific protein fragment can be released from the parent protein through gastrointestinal digestion or food processing, and exhibits a variety of biological functional activities, and plays an important role in the life activities of the body. effect. Biologically active peptides are formed by condensation of two or more amino acids, generally have 2 to 20 amino acid residues, and generally have a molecular weight of less than 6KDa. The structural characteristics (amino acid composition and sequence) of bioactive peptides affect the activity of active peptides, making bioactive peptides exhibit various biological activities, such as anti-o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/18C07K1/16A61K38/01A61P35/00
CPCA61K38/012C07K1/16C07K1/18C07K1/34C07K1/36C12P21/06
Inventor 刘智禹蔡康鹏吴靖娜蔡水淋
Owner FISHERIES RES INST OF FUJIAN
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