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A method for breeding special rice for pregnant women with high resistant starch and high zinc traits aggregated

A high-resistant starch and rice technology, applied in botany equipment and methods, plant gene improvement, application, etc., can solve problems such as impossible to meet zinc, achieve short breeding cycle, prevent and control diabetes in pregnant women, and promote zinc nutrition Effect

Active Publication Date: 2018-12-21
浙江绿巨人生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the average zinc content in ordinary rice sold on the market is only 10mg / Kg, and it is impossible to meet the demand for zinc when eating normally

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The breeding process of rice varieties for pregnant women with high resistant starch and high zinc traits is as follows:

[0025] (1) In the winter and spring of 2012, in Lingshui, Hainan, F 1 11 substitute seeds;

[0026] (2) Spring and summer of 2012, Hangzhou, Zhejiang, planting F 1 There are 11 plants, as many as possible backcross with parent II (Lilizi) once to obtain BC 1 f 1 129 seeds;

[0027] (3) In the winter and spring of 2013, Lingshui, Hainan, planted BC 1 f 1 Generation of 129 strains, using anther culture technology to construct a doubled haploid (DH) population;

[0028] (4) In the summer of 2013, DH was harvested per plant 0 Substitute seeds, construct 457 DH lines;

[0029] (5) In the winter and spring of 2014, Lingshui, Hainan, planting DH per plant 1 Line, harvested by individual plant, first select 92 plants with zinc content exceeding 30mg / kg, and then detect 25 plants with resistant starch content exceeding 10%;

[0030] (6) In the summ...

Embodiment 2

[0035] The breeding process of rice varieties for pregnant women with high resistant starch and high zinc traits is as follows:

[0036] (1) In the winter and spring of 2012, in Lingshui, Hainan, F 1 11 substitute seeds;

[0037] (2) Spring and summer of 2012, Hangzhou, Zhejiang, planting F 1 There are 11 plants, as many as possible backcross with parent II (Lilizi) once to obtain BC 1 f 1 129 seeds;

[0038] (3) In the winter and spring of 2013, Lingshui, Hainan, planted BC 1 f 1Generation of 129 strains, using anther culture technology to construct a doubled haploid (DH) population; the operation steps of the anther culture technology: before taking the ear, first scrub the flag leaf sheath with 70% alcohol. Peel off the inner sheath of the flag in the ultra-clean workbench, take out the rice ears, sterilize in 10% bleach solution for 10 minutes, wash once with sterile water, soak in 0.02% sodium tartrate solution for 10 minutes, and wash once with sterile water. Scru...

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Abstract

The invention discloses a breeding method of special high-resistance starch and high-zinc character polymerized rice for a pregnant woman. The breeding method comprises the steps of taking a high-resistance starch rice variety as a parent, performing hybridization with a high-zinc variety to obtain an F1 generation seed, performing backcrossing once with a glutinous rice variety for an F1 generation to obtain a BC1F1 generation seed, planting a BC1F1 plant, constructing a double haploid population by an anther culture technology, harvesting a DH0 generation seed according to a single plant, selecting a high-resistance starch and high-zinc-content nutrition character polymerized single plant for a DH1 generation, selecting a high-yield strain for a DH2 generation, selecting a strain with a moderate maturing period and a growing period not exceeding 140 days for a DH3 generation, and breeding a final strain for a DH4 generation to obtain a special rice variety for the pregnant woman. The special rice polymerizes excellent high-resistance starch and high-zinc characteristics; a technology of one backcrossing with the parent and following anther culture is adopted, so that the excellent comprehensive characteristic of the parent can be well reserved; the character is rapid and stable, and can be early evaluated.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding special rice for pregnant women with high resistant starch and high zinc traits aggregated. Background technique [0002] Rice is the traditional staple food of our country. Ordinary rice is too low in resistant starch and too high in digestible starch, which can easily lead to high blood sugar. The resistant starch in ordinary rice is generally less than 1%, and the content in high-quality rice is only below 0.5%. Therefore, increasing the resistant starch content in rice is the key. Breeding new rice varieties with high resistant starch content for diabetes is a research hotspot in the implementation of dietary prevention strategies. Gestational diabetes is a special type of diabetes and a backup condition of type 2 diabetes. Clinical data show that 2% to 3% of women will develop diabetes during pregnancy, mostly in the middle and late stages of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 徐开盛舒小丽徐望之
Owner 浙江绿巨人生物技术有限公司
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