A kind of head smut resistant sorghum breeding method
A head smut and sorghum-resistant technology, applied in the agricultural field, can solve problems such as low work efficiency, long time required, and large field area, and achieve the effects of accelerating breeding speed, strong operability, and reducing field breeding groups
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[0030] Example 1: Selection of R111*L17
[0031] R111 and L17 were crossed to obtain F1 seeds. The obtained F1 generation seeds were planted in the field, and the seeds of F3 generation were obtained by selfing two generations. Both generations of seeds were selected to resist head smut, and F4 generation seeds were harvested.
[0032] Indoor identification of resistance to head smut of F4 generation: Taking the resistance of single ear of F4 generation as a unit, 100 seeds per ear were taken for resistance identification indoor at a temperature of 25℃ and a relative humidity of 70%.
[0033] Plant sorghum seeds in a culture box filled with sand, inoculate with 0.5% fungal soil, and insert a card to mark the source. Thirty days after emergence, the seedlings were taken to peel off the outer leaves, leaving the growth cones, stained with aniline blue lactic acid stain, and microscopically examined whether the growth cone base tissues of the seedlings were stained with dark blue hyphae...
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