Method for breeding aphid-resistant sorghum

Abstract

The invention relates to a method for breeding aphid-resistant sorghum. The method comprises the following step of constructing an aphid-resistant hybrid population through hybridization and selfing of an aphid-resistant sorghum line serving as a female parent. By combining indoor seedling aphid resistance identification and field adult plant aphid resistance identification, the breeding method adopting twice aphid resistance identification and selection in one generation is realized, so that aphid-resistant sorghum varieties are bred rapidly and accurately.

Description

technical field

[0001] The invention relates to the field of agriculture, in particular to a breeding method for aphid-resistant sorghum. Background technique

[0002] Sorghum is the fifth largest grain crop in the world. It has been cultivated in my country for more than 5,000 years and was once widely planted as the main grain crop in my country. At present, sorghum is mainly grown in Jilin, Shanxi, Guizhou, Sichuan, Hebei and other places as raw grain for brewing wine and vinegar. Modern science has proved that the tannins and resistant starch in sorghum are beneficial to the human body, and have therapeutic effects on obesity, diabetes, hyperlipidemia and other chronic diseases. Eating sorghum is beneficial to health. Aphids harmful to sorghum in production include three types: sorghum aphid, wheat aphid, and corn aphid. The sorghum aphid is the most serious one for sorghum. This patent is only aimed at the sorghum aphid. Sorghum aphid is a common and rampant pest of s...

Examples

Embodiment 1

[0034] Embodiment 1: Stone R49 breeding

[0035] 130A and R47 were crossed to obtain F1 seeds: the obtained F1 seeds were planted in the field, and two generations of self-crossing were continuously planted to obtain F3 seeds, F2 aphid-resistant single plants were selected, and F3 seeds were harvested. Indoor identification of aphid resistance of the F3 generation: take the resistant single ear of the F3 generation as a unit, and take 100 seeds from each ear for resistance identification in a room with a temperature of 22-29°C and a relative humidity of 55-75%. The sorghum seeds are cultivated in the loam soil of the source field, the loam soil is packed in the nutrient hole of the nutrient tray, and the source is marked with a card. Aphids were bred 1 week in advance, and the number of bred aphids was more than 5 times the number of identified sorghum seedlings. At the 2-leaf stage, 3 aphids were artificially inoculated per plant. On the first day and the third day after in...

Embodiment 2

[0036] Embodiment 2: 98B breeding

[0037] 130B and 1041B were crossed to obtain F1 seeds: the obtained F1 seeds were planted in the field, and two generations of self-crossing were continuously planted to obtain F3 seeds, F2 aphid-resistant single plants were selected, and F3 seeds were harvested. Indoor identification of aphid resistance of the F3 generation: take the resistant single ear of the F3 generation as a unit, and take 100 seeds from each ear for resistance identification in a room with a temperature of 22-29°C and a relative humidity of 55-75%. The sorghum seeds are cultivated in the loam soil of the source field, the loam soil is packed in the nutrient hole of the nutrient tray, and the source is marked with a card. Aphids were bred 1 week in advance, and the number of bred aphids was more than 5 times the number of identified sorghum seedlings. At the 2-leaf stage, 3 aphids were artificially inoculated per plant. On the first day and the third day after inocul...

technical field

[0001] The invention relates to the field of agriculture, in particular to a breeding method for aphid-resistant sorghum. Background technique

[0002] Sorghum is the fifth largest grain crop in the world. It has been cultivated in my country for more than 5,000 years and was once widely planted as the main grain crop in my country. At present, sorghum is mainly grown in Jilin, Shanxi, Guizhou, Sichuan, Hebei and other places as raw grain for brewing wine and vinegar. Modern science has proved that the tannins and resistant starch in sorghum are beneficial to the human body, and have therapeutic effects on obesity, diabetes, hyperlipidemia and other chronic diseases. Eating sorghum is beneficial to health. Aphids harmful to sorghum in production include three types: sorghum aphid, wheat aphid, and corn aphid. The sorghum aphid is the most serious one for sorghum. This patent is only aimed at the sorghum aphid. Sorghum aphid is a common and rampant pest of s...

Examples

Embodiment 1

[0034] Embodiment 1: Stone R49 breeding

[0035] 130A and R47 were crossed to obtain F1 seeds: the obtained F1 seeds were planted in the field, and two generations of self-crossing were continuously planted to obtain F3 seeds, F2 aphid-resistant single plants were selected, and F3 seeds were harvested. Indoor identification of aphid resistance of the F3 generation: take the resistant single ear of the F3 generation as a unit, and take 100 seeds from each ear for resistance identification in a room with a temperature of 22-29°C and a relative humidity of 55-75%. The sorghum seeds are cultivated in the loam soil of the source field, the loam soil is packed in the nutrient hole of the nutrient tray, and the source is marked with a card. Aphids were bred 1 week in advance, and the number of bred aphids was more than 5 times the number of identified sorghum seedlings. At the 2-leaf stage, 3 aphids were artificially inoculated per plant. On the first day and the third day after in...

Embodiment 2

[0036] Embodiment 2: 98B breeding

[0037] 130B and 1041B were crossed to obtain F1 seeds: the obtained F1 seeds were planted in the field, and two generations of self-crossing were continuously planted to obtain F3 seeds, F2 aphid-resistant single plants were selected, and F3 seeds were harvested. Indoor identification of aphid resistance of the F3 generation: take the resistant single ear of the F3 generation as a unit, and take 100 seeds from each ear for resistance identification in a room with a temperature of 22-29°C and a relative humidity of 55-75%. The sorghum seeds are cultivated in the loam soil of the source field, the loam soil is packed in the nutrient hole of the nutrient tray, and the source is marked with a card. Aphids were bred 1 week in advance, and the number of bred aphids was more than 5 times the number of identified sorghum seedlings. At the 2-leaf stage, 3 aphids were artificially inoculated per plant. On the first day and the third day after inocul...

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