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A comprehensive fluorescence super-resolution microscopy imaging device

A microscopic imaging and super-resolution technology, applied in microscopes, measuring devices, fluorescence/phosphorescence, etc., can solve problems such as deficiencies, and achieve the effect of reducing the complexity of the system and the number of system modules

Active Publication Date: 2019-04-16
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For physical gratings and SLMs, the former requires a precision displacement servo system to translate and rotate them, while the latter is limited by the characteristics of SLM materials (mostly liquid crystals), and the time for them to obtain a frame of image is generally on the order of tens of ms. The highest shooting speed currently reported is about 100 frames, and the number of frames converted into super-resolution images is about 6 frames, which is still insufficient for the fast-changing biological movement process

Method used

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  • A comprehensive fluorescence super-resolution microscopy imaging device
  • A comprehensive fluorescence super-resolution microscopy imaging device
  • A comprehensive fluorescence super-resolution microscopy imaging device

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Embodiment Construction

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0037] The comprehensive fluorescence super-resolution microscopic imaging device of the present invention can obtain four coherent light beams through a beam splitting system, and arbitrarily adjust the incident position of the light beams at the entrance pupil of the microscope objective through the 4f galvanometer system, thereby realizing structured light illumination Microscopic imaging (SIM), three-dimensional structure light micros...

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Abstract

The invention discloses a comprehensive fluorescent and super-resolution microscopic imaging device. According to the invention, firstly, a light beam is divided into four symmetrical beams of light paths through three polarization beam splitters. Secondly, after the propagation angle and the position of the beams are modulated by a 4f-vibration mirror system, the light beams are combined through two beam splitters. Thirdly, the light beams are transmitted to the back focal plane of a microscope objective in the convergence manner through a 4f system. Finally, the four light beams are shot onto a sample in the parallel manner through the microscope objective and the beam interference occurs. Through the position adjustment of a shutter, a vortex phase plate and a scanning vibration mirror in the four beams of light paths, the latticed interference structure type light illumination mode, the horizontal strip-shaped interference structure type light illumination mode and the longitudinal strip-shaped interference structure type light illumination mode can be formed. Meanwhile, the light period, the phase and the direction of each structure can be arbitrarily and quickly adjusted. The device can realize the functions of structure light illumination microscopic imaging, three-dimensional structure light illumination microscopic imaging, annular total internal reflection fluorescence microscopic imaging, fourier frequency shift iterative microscopic imaging and the like.

Description

Technical field [0001] The invention relates to the field of microscopic imaging technology, in particular to a comprehensive fluorescent super-resolution microscopic imaging device. Background technique [0002] Cells are the basic structure and functional unit of all life activities. Exploring the life activities in cells can help people understand human life activities. Fluorescence super-resolution microscopy imaging technology has the advantages of non-destructive, strong specificity, and deep penetration depth. It can observe many life activities that occur in various organelles, such as gene transcription, protein synthesis and transportation, and material exchange. The research of resolution microscopy imaging technology and equipment is also endless, and has important scientific value and practical significance. [0003] At present, the mainstream super-resolution microscopy techniques include Stimulated Emission Depletion Microscopy (STED), Stochastic Optical Reconstruct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00G01N21/64
CPCG01N21/6458G02B21/0032G02B21/0056G02B21/0068G02B21/0076
Inventor 匡翠方陈友华朱大钊方月刘旭张克奇毛磊
Owner ZHEJIANG UNIV
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