The invention discloses a comprehensive fluorescent and super-resolution
microscopic imaging device. According to the invention, firstly, a
light beam is divided into four symmetrical beams of light paths through three polarization
beam splitters. Secondly, after the propagation angle and the position of the beams are modulated by a 4f-vibration mirror
system, the light beams are combined through two
beam splitters. Thirdly, the light beams are transmitted to the back focal plane of a
microscope objective in the convergence manner through a 4f
system. Finally, the four light beams are shot onto a sample in the parallel manner through the
microscope objective and the beam interference occurs. Through the position adjustment of a
shutter, a vortex
phase plate and a scanning vibration mirror in the four beams of light paths, the latticed interference
structure type light illumination mode, the horizontal strip-shaped interference
structure type light illumination mode and the longitudinal strip-shaped interference
structure type light illumination mode can be formed. Meanwhile, the light period, the phase and the direction of each structure can be arbitrarily and quickly adjusted. The device can realize the functions of
structure light illumination
microscopic imaging, three-dimensional
structure light illumination
microscopic imaging, annular
total internal reflection fluorescence microscopic imaging, fourier
frequency shift iterative microscopic imaging and the like.