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Application of lipopolysaccharide in respect of preparing additive for promoting animal feeding and improving composition of bodies

A lipopolysaccharide, animal body technology, applied in the application, animal feed, additional food elements and other directions, can solve the problems of poisoning and easy residue, and achieve the effects of simple preparation method, non-degradable and stable properties

Active Publication Date: 2017-09-05
沃曼(广东)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these "lean meat essence" substances are also easy to remain in meat and internal organs, and it is easy to cause various poisoning symptoms after human consumption

Method used

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  • Application of lipopolysaccharide in respect of preparing additive for promoting animal feeding and improving composition of bodies
  • Application of lipopolysaccharide in respect of preparing additive for promoting animal feeding and improving composition of bodies
  • Application of lipopolysaccharide in respect of preparing additive for promoting animal feeding and improving composition of bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Effect of adding lipopolysaccharide to drinking water on cumulative feed intake and expression of AgRP in arcuate nucleus of mice

[0029] In the experiment, 32 4-week-old male C57 / BL mice (13±1g) were selected and divided into 4 groups on average after pre-feeding. They were raised in single cages. One group was the blank control group, drinking water normally; mL of E.coil O128:B12 lipopolysaccharide; treatment group 2: add 5 µg / mL E.coil O127:B8 lipopolysaccharide to drinking water; treatment group 3: add 5 µg / mL E.coil O55:B5 lipopolysaccharide to drinking water. The above groups were given free access to food and water, and the mice were exposed to light for 12 hours, the ambient humidity was 70%±10%, and the temperature was kept constant at 23°C±1°C.

[0030] After feeding for 12 weeks, the cumulative food intake of the mice was detected. The results are attached figure 1 shown. It can be seen from the figure that adding 5 µg / mL LPS of different subty...

Embodiment 2

[0032] Example 2 Effects of different Escherichia coli subtype lipopolysaccharides added to drinking water on the body composition of mice

[0033] In the experiment, 32 4-week-old male C57 / BL mice (13±1g) were selected and divided into 4 groups on average after pre-feeding. They were raised in single cages. One group was the blank control group, drinking water normally; mL of E.coil O128:B12 lipopolysaccharide; treatment group 2: add 5 µg / mL E.coil O127:B8 lipopolysaccharide to drinking water; treatment group 3: add 5 µg / mL E.coil O55:B5 lipopolysaccharide to drinking water. In each of the above groups, the mice had free access to food and water, 12 hours of light cycle, the ambient humidity was 70%±10%, the temperature was constant at 23°C±1°C, and they were fed for 12 weeks.

[0034] At the end of the experiment, the fat content and lean tissue content of each mouse were measured with a MesoQMR23-060H nuclear magnetic resonance imager (Numei, Suzhou). After 12 weeks, the mi...

Embodiment 3

[0035] Example 3 Effects of different Escherichia coli subtype lipopolysaccharides on energy metabolism in mice in drinking water

[0036]In the experiment, 32 4-week-old male C57 / BL mice (13±1g) were selected and divided into 4 groups on average after pre-feeding. They were raised in single cages. One group was the blank control group, drinking water normally; mL of E.coil O128:B12 lipopolysaccharide; treatment group 2: add 5 µg / mL E.coil O127:B8 lipopolysaccharide to drinking water; treatment group 3: add 5 µg / mL E.coil O55:B5 lipopolysaccharide to drinking water. In each of the above groups, the mice had free access to food and water, 12 hours of light cycle, the ambient humidity was 70%±10%, the temperature was constant at 23°C±1°C, and they were fed for 12 weeks. After the experiment, the subcutaneous fat of the mice was cut and put into 4% formaldehyde solution for overnight fixation, then dehydrated by alcohol, transparentized in xylene, and embedded in paraffin; during...

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Abstract

The invention discloses an application of lipopolysaccharide in the respect of preparing an additive for promoting animal feeding and improving composition of bodies. Researches find that lipopolysaccharide from escherichia coli O128:B12,O127:B8,O55:B5 can notably improve the accumulated food consumption of animals, so that deposition of fat contents in the bodies of the animals can be notably reduced, besides, the lean tissue content of bodies of mice is increased, and the skeletal muscle content of the mice is increased. Besides, the subcutaneous fat content of the mice can also be notably reduced, the gastrocnemius content in the bodies of the mice is increased, and the metabolism level of brown fat is increased. Therefore, the escherichia coli is different from subtype lipopolysaccharide, and can improve the appetite of the animals and improve the quality of animal bodies; the lipopolysaccharide is free from poor reaction on the animal bodies, the lipopolysaccharide is stable in nature and not liable to degrade, the lipopolysaccharide can be directly added to drinking water or feeds, the addition process is convenient and swift, the preparation method is simple, special equipment requirements do not exist, and the lipopolysaccharide has good popularization and application prospects.

Description

technical field [0001] The invention belongs to the technical field of feed additives. More specifically, it relates to the application of lipopolysaccharide in the preparation of additives for promoting animal feed intake and improving carcass composition. Background technique [0002] Feed intake is an important means for animals to obtain nutrients and maintain metabolic balance in the body. Therefore, in the process of livestock and poultry production, the level of feed intake directly affects the performance of animal production. Studies have shown that animal feed intake is closely related to growth and development, reproduction, and lactation, and animal feed intake has become an important factor that limits its full production performance. Only by increasing the feed intake of animals to a certain extent can the production potential of animals be fully exerted. In addition, the modern aquaculture industry can ensure the nutritional needs of animals to the greatest ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163A23K20/158
CPCA23K20/158A23K20/163
Inventor 束刚余璐璐廖正睿汪滔朱灿俊袁业现蔡兴才朱晓彤王丽娜王松波高萍江青艳
Owner 沃曼(广东)生物科技有限公司
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