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Application of an osga3ox1 gene in the creation of rice male sterile lines

A male sterility and rice technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of unseen, high cost, deterioration of agronomic traits, etc., and achieve the effect of overcoming the high risk of seed production

Active Publication Date: 2021-10-08
江西省超级水稻研究发展中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still problems such as incomplete fertility, high cost, or serious deterioration of other agronomic traits in the preparation of metabolic-deficient sterile materials by genetic engineering methods, and there is no practical application of relevant results in production

Method used

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  • Application of an osga3ox1 gene in the creation of rice male sterile lines
  • Application of an osga3ox1 gene in the creation of rice male sterile lines
  • Application of an osga3ox1 gene in the creation of rice male sterile lines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1, a method of knocking out of a bibin synthesis gene OSGA3OX1 to create a rice urchinite system

[0068] The knockout of the OSGA3OX1 gene is completed by the following steps and obtain male infertility:

[0069] 1) Design SGRNA boot sequence

[0070] With online tool CRISPR-P (http: / / cbi.hzau.edu.cn / crisprus), the SG RNA boot sequence of the OSGA3OX1 gene is designed according to the method provided by the developer, and the sequence is based on the sequence from the results provided by the software. And the location of the sequence selects a suitable guidance sequence, the SGRNA boot sequence selected in this embodiment is the sequence shown in SEQ ID NO. 3 and SEQID No. 4.

[0071] 2) Synthesis of oligonucleic acids for cloning based on guidance sequences and carriers

[0072] This embodiment uses a carrier PCAMBIA1390 (constructed by non-profit mechanism CAMBIA), a carrier sequence and a map http: / / www.cambia.org / daisy / cambia / 585.html) CRISPR / Cas9 vector PCUB...

Embodiment 2

[0123] Example 2, OsGA3ox1 other fertility restoration gene knockout mutants

[0124] May be heading in rice species containing gibberellin GA through 8 a.m. 3 Spraying was panicle fertility restored, the concentration 45mg / L gibberellin, spraying four times, every 667m 2 The amount of 18g, gibberellin solution should contain an amount of a surfactant such as 0.01% Tween-20, to enhance adhesion of the agent in the blade surface, using the background kasalath when used at a concentration of about 30mg L GA / 3 When spraying ears just withdrawn.

Embodiment 3

[0125] Example 3, OsGA3ox1 knock restore fertility in addition mutants

[0126] May be heading in rice species containing gibberellin GA by 17:00 3 Spraying was panicle fertility restored, the concentration 60mg / L gibberellin, spraying once every 667m 2 The amount of 30g, gibberellin solution should contain an amount of a surfactant such as 0.03% Tween-20, to enhance adhesion of the agent in the blade surface.

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Abstract

The invention belongs to the technical field of rice breeding and relates to a method for creating male sterile strains using gibberellin synthetic genes. The creation of a sterile line is the key to the production of rice hybrids. In order to overcome the shortcomings of the existing "three-line" and "two-line" sterile lines, the invention provides a new type of sterile line and its creation method, specifically disclosing The invention relates to a method for creating a rice male sterile line by using the rice gibberellin synthetic gene OsGA3ox1 and a method for restoring the fertility of the sterile line. The invention provides a brand-new application of gibberellin synthetic gene OsGA3ox1, and provides a hybrid production that can reproduce itself by spraying gibberellin from exogenous sources. The fertility is not affected by environmental conditions, and overcomes the previous light and temperature A new type of dual-purpose sterile line system, which has the disadvantage of high risk of seed production due to the influence of extreme weather.

Description

Technical field [0001] The present invention belongs to the technical field rice breeding, to create a new method and application A related novel rice GA rice OsGA3ox1 gene synthesis infertility strains, particularly to rice OsGA3ox1 gene created in a male sterile line application, application methods create rice OsGA3ox1 gene and the fertility of male sterile lines created by this male-sterile rice lines recovery method. Background technique [0002] Obtain sterile line is the basis of heterosis in rice. Rice production is currently applied mainly for CMS cytoplasm (Cytoplasmic - Nuclear) male sterile line and a light PTGMS Line. Related seed accordingly divided into "three-line" and "two-line" Seed. "Three Lines" system due to the presence of coordination group is not free of problems restoring and maintaining relationship restrictions, and because fertility restoration of non-simply by single genes, increased recovery system or keep the difficulty of the Department of cultivat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02A01G7/06A01H1/02A01H5/00A01H6/46
CPCA01G7/06A01H1/02C12N9/0071C12N15/8213C12N15/8289C12Y114/11015
Inventor 龙起樟万建林黄永兰王会民唐秀英芦明
Owner 江西省超级水稻研究发展中心
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