A method for isolation and identification of noni endophytic fungi

Abstract

The invention relates to a separation and identification method for endophytic fungi of morinda citrifolia and belongs to the technical field of microorganism separation and identification. The separation and identification method for endophytic fungi of morinda citrifolia provided by the invention comprises the following steps: 1) performing surface sterilization on morinda citrifolia fruits and leaves; 2) placing inner tissues of the morinda citrifolia fruits and leaves onto a potato dextrose agar culture medium and culturing for 8-10 days under a dark condition at 25+ / -1 DEG C; 3) performing subculture on the strain cultured and acquired in the step 2) and purifying by performing 4-5 times of continuous hypha tip transfer, thereby acquiring the endophytic fungi of morinda citrifolia; and 4) performing ITS sequencing identification on the endophytic fungi of morinda citrifolia acquired in the step 3). According to the method provided by the invention, the effective separation and identification for the endophytic fungi of morinda citrifolia can be realized, the separated endophytic fungi is utilized to produce the corresponding active material through a fermentation technique, the basis is established for researching the drug action of the endophytic fungi in morinda citrifolia and a new thought is supplied for researching and developing the antitumor and anti-inflammation drugs.

Description

technical field [0001] The invention relates to the technical field of microorganism isolation and identification, in particular to a method for isolation and identification of noni endophytic fungi. Background technique [0002] Endophytic fungi refer to a class of beneficial fungi that live in aboveground and living plant tissues, but do not cause disease in the host plant. Therefore, plant endophytic fungi are natural components of the micro-ecosystem in plants. They live in this special environment in plants for a long time and will not cause plant diseases. Form a symbiotic relationship. The secondary metabolites of endophytic fungi in medicinal plants are very rich, and have biological activities such as antibacterial, antitumor, antiviral, insecticidal, immune regulation, and plant growth promotion. It is one of the effective ways to screen bioactive components or lead compounds. It has potential application value in industrial fermentation, biopharmaceutical, agric...

AI Technical Summary

Problems solved by technology

At present, endophytic fungi are often separated by tissue separation, and the defects are: (1) excessive surface disinfection, killing some endophytic fungi; (2) insufficient surface disinfection, surface bacterial contamination; (3) fast-growing endophytic fungi Cover some slow-growing fungi; (4) The medium used does not ensure the growth of all endophytic fungi

The medicinal potential of noni endophytic fungi has not been tapped to a great extent

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