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Diagnostic kit for detecting peripheral blood protein marker of Alzheimer disease and detection method using diagnostic kit

A technology for Alzheimer's disease and protein markers, applied in the field of in vitro diagnostic testing, can solve the problems of poor repeatability, insufficient sensitivity, and poor sensitivity of solid-phase biochip technology, and achieve high sensitivity, good stability, and detection quick effect

Active Publication Date: 2017-10-10
WUXI MENTAL HEALTH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies can only detect one marker at a time, and the operation is cumbersome and the sensitivity is poor, which cannot really meet the needs of clinical diagnosis and detection.
However, solid-phase biochip technology has disadvantages such as poor reproducibility, insufficient sensitivity, and cumbersome operations.

Method used

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  • Diagnostic kit for detecting peripheral blood protein marker of Alzheimer disease and detection method using diagnostic kit
  • Diagnostic kit for detecting peripheral blood protein marker of Alzheimer disease and detection method using diagnostic kit
  • Diagnostic kit for detecting peripheral blood protein marker of Alzheimer disease and detection method using diagnostic kit

Examples

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Embodiment 1

[0047] Example 1: Liquid-phase chip combined parallel detection method for 9 kinds of Alzheimer's disease markers. The specific detection method includes the following steps:

[0048] 1. Activation of required microspheres

[0049] 1.1 Vortex the microsphere storage solution at full speed for at least 3 minutes to form a uniform microsphere suspension;

[0050] 1.2 Weigh 10mg of EDC and S-NHS into two centrifuge tubes;

[0051] 1.3 Dissolve with deionized water to make the final concentration 50mg / mL;

[0052] 1.4 Centrifuge 1mL of microsphere suspension at 10000g for 3min, and carefully remove the supernatant;

[0053] 1.5 Add 80μL of activation buffer to resuspend the microspheres;

[0054] 1.6 Add 10μL of EDC solution (50mg / mL) and 10μL of S-NHS solution (50mg / mL) respectively, mix well, room temperature (15-25℃), avoid light, and incubate with shaking for 20min.

[0055] 2. The corresponding capture antibody is coupled to the activated microsphere

[0056] 2.1 Use coupling buffer to dil...

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Abstract

The invention relates to a diagnostic kit for detecting a peripheral blood protein marker of an Alzheimer disease and a detection method using the diagnostic kit and belongs to the technical field of in vitro diagnosis detection. The detection method is characterized in that various protein markers in a same sample can be detected at one time, namely, a tetrad compound 'biotin-labeled detection antibody-Alzheimer disease protein marker-capture antibody-microsphere' is formed in the manner of preparation of a liquid chip, and fluorescence signals of different microspheres can be detected after the tetrad compound is combined with streptavidin-phycoerythrin, so that the existence and content of various protein markers related to the Alzheimer disease in a to-be-detected sample can be confirmed. The invention also discloses the components forming the diagnostic kit. The method and the kit provided by the invention have the advantages of high sensitivity, high throughput, high detection speed, accuracy, and the like, and can be used for simultaneously performing qualitative and quantitative detection on various protein markers related to the Alzheimer disease.

Description

Technical field [0001] The invention relates to a diagnostic kit for detecting Alzheimer's disease peripheral blood protein markers and a detection method thereof, and belongs to the technical field of in vitro diagnostic detection. Background technique [0002] Alzheimer's disease (AD) is a progressive neurodegenerative disease with insidious onset. Clinically, it is characterized by general dementia such as memory impairment, aphasia, apraxia, cognition, impairment of visual spatial skills, executive dysfunction, and personality and behavior changes. The etiology is still unknown. Because Alzheimer is a degenerative disease of the central nervous system that progresses slowly, deteriorates over time and is irreversible, patients usually progress from early recent memory loss to loss of physical function, and eventually death. AD not only threatens lives directly, but also causes a serious decline in the quality of life. The associated loss of labor and care costs are even more...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/533G01N33/68G01N2800/2821
Inventor 程灶火
Owner WUXI MENTAL HEALTH CENT
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