Cartilaginous fish skeleton specimen making method

A kind of specimen making and fish technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of cumbersome production process, cartilage deformation, wrong bone position assembly, etc., to increase learning interest and have broad market prospects. , the effect of maintaining integrity

Inactive Publication Date: 2017-10-20
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

There are two traditional methods for making fish bones: one is to remove all the muscle parts of the fish and reassemble the bones to their original state. The production process is cumbersome, and it is easy to lose some small bones or the bones are assembled incorrectly. The production of bone specimens

Method used

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Embodiment Construction

[0014] Specific embodiments of the present invention will be described below.

[0015] A kind of cartilaginous fish skeleton dyeing plastic specimen preparation method, its condition and step are:

[0016] Fish body preparation: select fresh and complete fish body of cartilaginous fish, remove internal organs, scales and fish skin, soak in 10% formalin fixative solution, store for 2-3 days or for a long time, if the specimen has been stored in formalin Marin can be soaked directly in distilled water. Soak the fish body in distilled water for 1-2 days to remove formalin.

[0017] Cartilage staining: Put the fish body into the mixed dye solution (0.1g Alcian blue, 75-80ml 95% ethanol and 20-25ml glacial acetic acid, the mixed dye solution is ready-to-use) and stained for 2-3 days.

[0018] Osmolarity adjustment: Then transfer to 95% alcohol, change alcohol after 1-2 hours. Then transfer to a series of different concentrations of alcohol, 70%, 40%, 15% alcohol, each concentrat...

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PUM

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Abstract

The invention discloses a making method for a skeleton-dyed specimen plastic package specimen of cartilaginous fish. The making method comprises the following conditions and steps: cleaning internal organs, scales and the skin of a fish body, fixing the fish body with 10 percent of formalin, soaking the fish body with distilled water for 1 to 2 days to make sure that the formalin is completely cleared away, dyeing the skeleton and cartilages into blue with a dye alcian blue, performing treatment with alcohol at different concentrations, then treating muscles of the fish body with trypsin till a transparent state is achieved, retaining the blue color in the cartilages, dyeing hard bones into red with alizarin red, performing transparent treatment again with 0.5-percent KOH and glycerinum mixed solution at different ratios, then plasticizing the fish body with polyvinylpyrrolidone, after the fish body is plasticized, drying the specimen in air, and finally sealing the specimen with epoxy resin. According to the completed specimen, the skeleton specimen in which the hard bones are red and the cartilages are blue can be seen directly through the resin and the plasticized muscles.

Description

technical field [0001] The invention relates to a method for preparing cartilaginous fish skeleton specimens, in particular to a method for preparing cartilaginous fish skeleton dyed plastic-encapsulated specimens. Background technique [0002] Fish skeleton specimens are part of ichthyology teaching and an important part of popular science education. Cartilaginous fishes are an important part of fish. Fish skeleton specimens can help students and children learn the knowledge of fish skeletal system. There are two traditional methods for making fish bones: one is to remove all the muscle parts of the fish and reassemble the bones to their original state. The production process is cumbersome, and it is easy to lose some small bones or the bones are assembled incorrectly. The production of bone specimens The efficiency is low, and the cartilage will deform after dehydration, and such fish skeleton specimens also reduce the significance of education. The second is to store th...

Claims

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Application Information

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IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 张赛赛王伟姜欣彤
Owner DALIAN OCEAN UNIV
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