Building method of animal model of ruptured lumbar intervertebral disc protrusion

Abstract

The invention discloses a building method of an animal model of ruptured lumbar intervertebral disc protrusion. The method comprises the steps of cutting a first caudal vertebra intervertebral disc and a second caudal vertebra intervertebral disc of a rat, and cutting upper and lower cartilage endplates of the first caudal vertebra intervertebral disc to make nucleus pulposus exposed; piercing lateral annulus of the second caudal vertebra intervertebral disc, and fishing out nucleus pulposus of the annulus; embedding the first caudal vertebra intervertebral disc in muscles outside canalis vertebralis of the rat to make the nucleus pulposus be in contact with blood supply; implanting the second caudal vertebra intervertebral disc into lumbar vertebral canalis vertebralis of the rat to compress the base of nerve roots of the rat. By means of the building method of the animal model of the ruptured lumbar intervertebral disc protrusion, simulation of the fact that intervertebral disc nucleus pulposus is in sufficient contact with the blood supply after the posterior longitudinal ligament rupture is a breakthrough point, through a method of artificially exsecting the exposed nucleus pulposus of the cartilage endplates, the condition for exposing the nucleus pulposus tissue in blood circulation and body immunity systems is created, and the animal model which can promote reabsorption of the protruding intervertebral discs is built.

Description

technical field [0001] The invention relates to a method for establishing an animal model, in particular to a method for establishing an animal model for ruptured lumbar disc herniation. Background technique [0002] In recent years, with the continuous deepening of people's understanding of lumbar disc herniation (LDH), people hope that by establishing a reliable and effective LDH animal model and applying intervention factors, the changes in the model can be observed more accurately. Explore the pathogenesis of diseases in the human body, and promote the research results to the clinic, and provide reasonable and effective prevention and treatment methods for the treatment of lumbar disc herniation. Therefore, the establishment of an ideal LDH animal model is of great significance for the mechanism research and clinical prevention and treatment of this disease. [0003] At present, lumbar disc herniation is divided into unruptured type and ruptured type according to whethe...

AI Technical Summary

Problems solved by technology

[0006] In the current experiments on the reabsorption of intervertebral disc herniation, domestic and foreign scholars mostly use large animals such as pigs and rabbits or adopt cell culture models in vitro, but there is no mature and effective model available for the establishment of rat models

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