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Detection method for mouse mouth dryness and application of detection method

A detection method and mouse technology, which can be used in testing food, measuring devices, testing beverages, etc., can solve problems such as large workload, data errors, and poor experimental controllability, and achieve simple operation, intuitive expression, and anti-environmental interference ability. strong effect

Inactive Publication Date: 2017-12-01
JING BRAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, the consideration of dry mouth in preclinical animal experiments and clinical studies, such as the use of subjective evaluation scales, is a qualitative investigation, often due to subjective factors leading to large data errors; such as the use of saliva secretion, saliva flow rate and dietary changes amount, the controllability of the experiment is relatively poor, and the workload is large

Method used

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  • Detection method for mouse mouth dryness and application of detection method
  • Detection method for mouse mouth dryness and application of detection method
  • Detection method for mouse mouth dryness and application of detection method

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Experimental program
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Effect test

Embodiment 1

[0038] The present embodiment provides a method for detecting the degree of mouth dryness in mice after drinking water, which comprises the following steps:

[0039] (1) Male BALB / c mice with good adaptive feeding were selected, and all mice were fasted and had free access to water 12 hours before gavage.

[0040] (2) All mice were randomly divided into 5 groups, 10 in each group, respectively: "water deprivation 0 hour" group, "0.5 hour after gavage" group, "1 hour after gavage" group, "after gavage 2 hours" group, "3 hours after gavage" group. All the mice were given oral gavage with purified water in turn and water was withheld, and the time of gavage was recorded well. The volume of gavage for mice was 0.19mL / 10g.bw.

[0041] (3) The mice in the "water deprivation 0 hour" group were immediately plucked eyeballs to take blood after gavage, and the mice in the "0.5 hour after gavage" group were plucked eyeballs to get blood 0.5 hours after gavage. The mice in the "1 hour a...

Embodiment 2

[0049] This embodiment provides a method for detecting the degree of dry mouth in mice after drinking, which is used to further verify the relationship between the serum osmotic pressure of mice and the degree of dry mouth in mice, which includes the following steps:

[0050] (1) Male BALB / c mice with good adaptive feeding were selected, and all mice were fasted and had free access to water 12 hours before gavage.

[0051] (2) All mice were randomly divided into 5 groups, 10 in each group, respectively: "0.5 hour after water gavage" group, "0.5 hour after alcohol gavage" group, "1 hour after alcohol gavage" group, "2 hours after alcohol gavage" group and "3 hours after alcohol gavage" group. Mice in the "0.5 hour after water gavage" group were given pure water gavage and water deprivation, mice in other groups were given alcohol gavage and water deprivation, and the time of gavage was recorded. The volume of gavage of mice was 0.19mL / 10g.bw.

[0052] (3) The mice in the "0.5...

Embodiment 3

[0060] This embodiment provides a method for detecting the degree of dry mouth in mice after drinking, which is used to further verify the relationship between the serum osmotic pressure of mice and the degree of dry mouth in mice, which includes the following steps:

[0061] (1) Male BALB / c mice with good adaptive feeding were selected, and all mice were fasted and had free access to water 16 hours before gavage.

[0062] (2) All mice were randomly divided into 7 groups, 10 in each group, respectively: "0.5 hour after water gavage" group, "0.5 hour after alcohol gavage" group, "1 hour after alcohol gavage" group, "1.5 hours after alcohol gavage" group, "2 hours after alcohol gavage" group, "2.5 hours after alcohol gavage" group and "3 hours after alcohol gavage" group. Mice in the "0.5 hour after water gavage" group were given pure water gavage and water deprivation, mice in other groups were given alcohol gavage and water deprivation, and the time of gavage was recorded. The...

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Abstract

A method for detecting dry mouth of mice and its application, relating to the technical field of alcohol pharmacology research, the detection method is as follows: the mice are randomly divided into several groups, and all the mice are gavaged and deprived of water; Eyeballs were picked from the mice to take blood, and the osmotic pressure of the blood samples of the mice in each group was measured; according to the osmotic pressure of the blood samples of the mice in each group, the degree of dry mouth of the mice after gavage was judged. The higher the osmotic pressure, the degree of dry mouth more obvious. The method is easy to operate, intuitive in expression, high in detection sensitivity, and strong in anti-environmental interference, and can quickly and effectively evaluate the degree of dry mouth in mice after drinking. The detection method can be used to detect the change of the degree of dry mouth of mice to a kind of wine or the difference of the degree of dry mouth of mice to different wines, so as to obtain the degree of dry mouth caused by wine or the difference of the degree of dry mouth caused by different wines.

Description

technical field [0001] The invention relates to the technical field of alcohol pharmacology research, and in particular to a method for detecting dry mouth in mice and an application thereof. Background technique [0002] Dry mouth, or xerostomia, refers to the state or feeling of dry mouth caused by decreased saliva secretion, and is an oral symptom with multiple triggering factors. Although dry mouth is only a symptom, it can cause secondary diseases, and even affect the quality of life of patients in severe cases. There are many factors that lead to dry mouth, including various clinical diseases and changes in physiology, psychology, nerves, endocrine, and the application of therapeutic methods or drugs. [0003] Dry mouth after drinking is a common phenomenon reported by various alcohol consumers. How to reduce or even avoid dry mouth after drinking alcohol products has become the core interest point of the competition of alcohol companies. In the process of product de...

Claims

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Application Information

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IPC IPC(8): G01N33/14
CPCG01N33/146
Inventor 石姣陆世广刘源才杨跃军单义民石莹莹张亚方陈默思王磊刘胜华明露
Owner JING BRAND
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